A process for isolating cytoplasmic proteins from aqueous vegetable materials containing chloroplastic and cytoplasmic proteins which comprises adding chitosan to the vegetable material to form a flocculate of the chloroplastic proteins and a supernatant, separating the flocculate and recovering a fraction ...
Peptides play a key role in plant development and intercellular communication. A variety of novel peptides and peptidic compounds have been isolated over the last few decades. This chapter examines the various methods used to isolate and identify new peptides and peptidic compounds from plants, and...
to isolate DNA from soil (MoBio laboratories, Carlsbad, CA, USA). We quantified extracted DNA using the Qubit dsDNA High-Sensitivity assay kit and a Qubit 2.0 Fluorometer (Invitrogen, Eugene, OR, USA). To quantify the degree of18O isotope incorporation into bacterial DNA, we performed ...
Here, we present the results of a genome-wide screen for cell envelope-associated resistance and susceptibility determinants in a Burkholderia cenocepacia clinical isolate. For this purpose, we construct a high-density, randomly-barcoded transposon mutant library and expose it to 19 cell envelope-...
Using next-generation sequencing approaches to isolate simple sequence repeat (SSR) loci in the plant sciences. (Special Issue: Methods and applications of... The application of next-generation sequencing (NGS) technologies for the development of simple sequence repeat (SSR) or microsatellite loci ...
solanacearum isolate was obtained from the Institute of Plant Protection, Henan Academy of Agricultural Sciences. The bacteria were streaked on 1% TTC agar medium (containing 10.0 g L− 1 peptone, 1 g L− 1 casein hydrolysate, 10.0 g L− 1 D-glucose, and 18.0 g L− 1 ...
On one hand, the molecular diversity of this collection was assessed by using 10 individuals per accession screened with 21 microsatellites and a DNA marker for phaseolin-type [3]. Then, 14 traits related to seed and plant morphology were also characterized [3]. On average, the collection ...
In the study of transposable elements (TEs), the generation of a high confidence set of consensus sequences that represent the diversity of TEs found in a given genome is a key step in the path to investigate these fascinating genomic elements. Many algo
1f). We then conducted a forward genetic screen to isolate suppressors of atm under TOP1i (Fig. 3a). Among isolated atm short-root recovery (asr) mutants, we focused on the first isolated suppressor mutant asr20, whose root length was significantly recovered in CPT treatment when compared ...
Traditional plant varieties that have been developed through informal (farmer-based) breeding. Germplasm Living material, such as seeds or tissues, from which new plants can be grown that is maintained for the purpose of preservation, breeding and other uses. Durability A property that enables resis...