Key Laboratory of Plant Germplasm Enhancement and Specialty AgricultureBo WangGraduate University of Chinese Academy of SciencesLei PanJunhua Peng遗传学报:英文版Zhang L, Wang B, Pan L, Peng J (2013) Recycling isolation of plant DNA, a novel method. J Genet Genomics 40(1):45–54. : 10....
Isolation of Plant DNA from fresh tissue Isolation of plant DNA from fresh tissue DOYLE JJ Focus 12, 13-15, 1990 JJT Doyle,JL Doyle - 《Focus》 被引量: 1.5万发表: 1990年 A rapid total DNA preparation procedure for fresh plant tissue Isolation of plant DNA from fresh tissue DOYLE JJ ...
V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6531–6535. Article PubMed CAS Google Scholar Tai, T. H. and Tanksley, S. D. (1990) A rapid and inexpensive method for isolation of total DNA from dehydrated plant tissue....
A general difficulty in isolation of DNA from plant cells is the presence of a cell wall. It is necessary to degrade plant cell walls, either physically or enzymatically, in order to effectively isolate plant DNA. Additionally, some tissues (such as endosperm) or some species contain high leve...
Unlike the animal cell, DNA extraction from plant cells faces challenges of a rigid cellulose cell wall and the presence of a variety of cytoplasmic components like polysaccharides, polyphenols, lipids, and proteins. This demands modification in the DNA
The 96 Well Synergy™ Plant DNA Extraction Kit is a bead beating based kit, allowing for high throughput sample processing. Impurities are captured on the Synergy™ grinding matrix and DNA in the lysate is further purified using filter plates. Unlike most commercially-available kits, the ...
在我们的PowerPlant Pro DNA和RNA提取试剂盒都是用了特殊的溶液Phenolic Separation Solution (PSS),可以在均质样品前就加入到研磨管中。它能非常高效地抑制多酚活性。但我们也发现对某些样品能显著提高核酸得率,而对另一些样品却似乎毫无效果。可能跟植物种类有关系。所以建议先做个对比测试,以决定你的样品是否需要添...
Isolation of plant DNA from fresh tissue Rapid isolation of high molecular weight plant DNA. Isolation of putative progenitor endothelial cells for angiogenesis. Isolation of putative progenitor endothelial cells for angiogenesis. A rapid DNA isolation procedure for small quantities of fresh leaf tissue ...
All DNAs are suitable for digestion with restriction endonucleases, ligation, and cloning in Escherichia coli, and DNAs from step three are recovered in high-molecular-weight form. The procedure has been used successfully with several dozen isolates of the plant pathogenic fungus Cochliobolus heteros...
DNA isolation from some fungal organisms of agronomic importance is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. We have developed a fast DNA isolation protocol for Fusarium oxysporum, which causes fusarium wilt disease in more than 100 plant species,...