Scaling the procedure down 10-fold results in a miniprep method that is very suitable for extracting small quantities (25–100 µg) of DNA from young, fresh leaves.doi:10.1385/0-89603-254-x:9J StaceyP G IsaacHumana PressStacey J, Isaac P (1994) Isolation of DNA from plants. In: ...
Isolation of DNA from plants This chapter describes a DNA extraction method that can be used both on freeze-dried leaves and on fresh leaves, and is based on the method of Saghai-Maroof ( 1 ), modified by David Hoisington and Jack Gardiner at the University of Misso... J Stacey,P G...
2007. A rapid efficient method for DNA isolation from plants with high levels of secondary metabolites. Asian J. Plant Sci. 6(6): 977- 981DEHESTANI, A.; TABAR S. K. K. A rapid efficient method for DNA isolation from plants with high levels of secondary metabolites. Asian Journal of ...
The effectiveness of application of FP-PANI-sorbent for single-step isolation of NAs from the samples of different origin (viruses, prokaryotes, eukaryotes) and various type (bacterial cultures, biological fluids, tissues of fungi, plants, and animals, food products, etc.) using different ...
plants, and bacteria species. For these organisms, it is recommended to include an extra step for cell lysis before disrupting the plasma membrane. This step is usually an enzymatic or mechanical process to disrupt the cell wall. The purification of DNA and RNA molecules is required to avoid ...
The PureLink HiPure Plasmid Filter Purification Kits allow isolation of high yields of highly pure plasmid DNA. The HiPure Filter Column enables rapid clearing of the bacterial lysate without the need for centrifugation. The lysate Filtration Ca...
The process starts with the extraction of DNA directly from the environmental samples, followed by library preparation and HTS. The quality of DNA is crucial in generating high-quality data for HTS-based microbial community analysis. DNA isolation from some environmental samples can be challenging ...
(mddcc) individuals were isolated from the progeny of these plants. We named themet1allele generated in the quadruple mutant backgroundmet1-8(Fig.1aand Supplementary Fig.1a). In parallel, the same CRISPR/Cas9 vector was used to produce amet1single mutant; the allele generated in the WT ...
The codon-adapted fusion proteins of stilbene synthase and 4-coumaroyl-CoA ligase in the last two steps of resveratrol synthesis in higher plants are fused with a glycine-serine-glycine tripeptide linker (Roy et al., 1997). The fusion protein was overexpressed in the wild type Chlamydomonas ...
Leaves of 21-day-old plants were harvested to extract intact chloroplasts using a chloroplast isolation kit (Invent, CP-011) following the instructions from the manufacturer. Briefly, plant leaves were added to a filter column containing 200 µl cold buffer A, and were gently ground with a...