Lachova M, Lehotay J, Karasova G, Skacani I, Armstrong DW. Isolation of L-theanine from plant material using a molecularly imprinted polymer. J Liq Chromatogr Relat Technol 2007;30:2045-58.Isolation of L-theaninefrom plant material using a molecularly imprinted polymer. M. Lachova?,J. ...
The NucleoSpin 8 Plant II and NucleoSpin 96 Plant II kits enable time-saving parallel isolation of genomic DNA from plant cells and tissue in a flexible 8-well strip format and a convenient 96-well plate high-throughput format, respectively. The kits are suitable for processing...
DNAzol™ Reagent is a complete and ready-to-use organic reagent for the isolation of genomic DNA from solid and liquid samples of animal, plant, yeast, and bacterial origin. The DNAzol™ Reagent procedure can be completed in typically 10–30 minutes with DNA recoveries of 70–100%. DNA...
The NucleoSpin Food kit allows you to quickly isolate genomic DNA from many types of processed food or animal feed of plant or animal origin. Food samples are highly heterogeneous and contain many different compounds such as fats and polysaccharides. These characteristics make DNA extraction ...
amounts of cpDNA and mtDNA in the DNA sample. This resulted in 60-fold coverage of the barley chloroplast genome and only ~10% of the barley nuclear genome being sequenced [12]. In general, plant genome projects aim for below 10% organellar contamination when plant material permits to ...
(Optional) For large pellets containing >200 μg DNA or large amounts of a non-DNA material, repeat steps a–d a third time. Wash the pellet with 1.5–2 ml of 75% ethanol for 10–20 min at room temp This ethanol wash removes pinkish ...
Various problems are encountered during DNA extraction from plant species harbouring high levels of secondary metabolites and polysaccharides. Trochetia species are known to synthesize a wide spectrum of polyphenols including flavonoids. These compounds represent a significant barrier to the extraction of pu...
For any molecular studies the isolation of pure, intact, and high-quality DNA is very crucial. However, secondary metabolites by excessive contamination interfere with DNA isolation. Unlike animals and microbes, plants contain a variety of secondary metabolites. These metabolites vary from plant to pl...
NucleoSpin 8/96 Food Kits: Isolate genomic DNA from processed food or animal feed of plant or animal origin in a high-throughput format. We have optimized the lysis buffer and purification process to extract DNA from a variety of foods.
Starting Material AmountBacteria: ≤107cells Cells: ≤107 Plant: ≤250 mg Tissue: ≤250 mg Virus: ≤107cells Yeast: ≤107cells Enzymatic Reactions: varies YieldCells: ≤16 μg RNA per 50,000 cells Tissue: ≤160 μg per 5 mg Unit SizeEach ...