FIELD: microbiology.;SUBSTANCE: invention relates to molecular biology, in particular to a method for isolating DNA from plants, suitable for PCR. Sample under study is homogenized in a non-extracting buffer containing TE buffer and polyvinylpyrrolidone (PVP). Next, perform primary lysis using ...
An improved method of DNA isolation from plants collected in the field and conserved in saturated NaCL/ CTAB solution. Taxon 49, 79 - 84.Sˇ torchova, H., Hrdlicˇkova, R., Chrtek, J., Tetera, M., Fitze, D. & Fehrer, J. (2000) An improved method of DNA isolation from plants...
A rapid efficient method for DNA isolation from plants with high levels of secondary metabolites. Asian J. Plt. Sci. 6(6): 977-981.Dehestani A and Tabar SKK (2007). A rapid efficient method for DNA isolation from plants with high levels of secondary metabolites. Asian Journal of Plant ...
NON-DESTRUCTIVE PROCEDURE FOR THE ISOLATION OF DNA FROM PLANTS Provided is a method for obtaining DNA from a plant by collecting the root border cells from a growing root and extracting DNA from the root border cells. ... CMP Van Dun 被引量: 0发表: 2009年 [Methods in Enzymology] Molecula...
Current DNA isolation methods are limited in their ability to obtain quality and/or quantity DNA from plants, such as Emblica officinalis, Terminalia belerica, and Terminalia chebula, which have low pH and high amounts of secondary metabolites in tissue extracts. Our modified DNA isolation method ...
high molecular weight nuclear DNA from several species of plants with reduced levels of cpDNA and mtDNA contamination. We also developed a method that uses quantitative real time PCR (qPCR) to determine the level of cpDNA and/or mtDNA contamination in plant DNA preps. The DNA isolation protocols...
CTAB Total DNA Isolation The high polysaccharide content of some plant species hinders the successful isolation of their DNA. As an alternative to the macro-extraction methods prev... J Doyle 被引量: 57发表: 1991年 Optimising DNA isolation for medicinal plants In African traditional health care ...
DNA and RNA Isolation Techniques for Non-Experts Akash Gautam Part of the book series:Techniques in Life Science and Biomedicine for the Non-Expert((TLSBNE)) Abstract Unlike the animal cell, DNA extraction from plant cells faces challenges of a rigid cellulose cell wall and the presence of a...
A Rapid Efficient Method for DNA Isolation from Plants with High Levels of Secondary Metabolites This research was designated to optimize a rapid, inexpensive method to isolate desirable DNA from plants with high concentrations of secondary products. D... A Dehestani,SKK Tabar - 《Asian Journal ...
Isolation, characterization and distribution of a DNA ligase from higher plants 来自 Semantic Scholar 喜欢 0 阅读量: 27 作者: B Kessler 摘要: 1. A DNA ligase has been isolated from various organs and subcellular components of plants. It has been purified 600-fold from pea roots. 2. The ...