Hawkins TL, O'Connor-Morin T, Roy A et al. (1994) DNA purification and isolation using a solid-phase.Nucleic Acids Res22(21):4543-4544. Thermo Fisher Scientific, Inc. (2018) Qubit dsDNA assay specificity in the presence o...
plants, and bacteria species. For these organisms, it is recommended to include an extra step for cell lysis before disrupting the plasma membrane. This step is usually an enzymatic or mechanical process to disrupt the cell wall. The purification of DNA and RNA molecules is required to avoid ...
DNA in cells is supercoiled and constrained into loops and this supercoiling and looping influence every aspect of DNA activity. We show here that negative supercoiling transmits mechanical stress along the DNA backbone to disrupt base pairing at specifi
While various clinical applications especially in oncology are now in progress such as diagnosis, prognosis, therapy monitoring, or patient follow-up, the
Therefore, DNA isolation methods need to be adjusted according to the nature of metabolites. Several protocols have been developed for obtaining the best quality and highest yield of DNA from plants containing high levels of secondary metabolites. Some plants (e.g., mangrove and salt marsh) are ...
Target protein-oriented isolation of Hes1 dimer inhibitors using protein based methods Midori A. Arai Kaori Morita Masami Ishibashi Scientific Reports (2020) Screening for natural inhibitors of human topoisomerases from medicinal plants with bio-affinity ultrafiltration and LC–MS Minxia Fan Guilin ...
Isolation of nucleic acidPlant seedsCetyl trimethylammonium bromideCTABMolecular techniquesQuality of nucleic acidsPlants’ genomic DNAGMO detectionBackground Reliable measurement of DNA concentration and purity is important for almost all molecular genetics studies. Different plant species have varying levels ...
In complex eukaryotic cells such as those from plants, animals, fungi and protists, most of the DNA is located in the cell nucleus. By contrast, in simpler cells called prokaryotes, including the eubacteria and archaea, DNA is not separated from the cytoplasm by a nuclear envelope. The ...
using an Easy Yeast Plasmid Isolation Kit (PT4073–1, Clontech), and was transformed into DH5αEscherichia coli. The recombined pGADT7-Rec2-ERF2 vector was isolated fromE. ColiusingE.Z.N.A.TM Plasmid Mini Kit (OMEGA). Screening the random DNA insertion library...
F. Isolation of cloned Moloney murine leukemia virus reverse transcriptase lacking ribonuclease H activity. Nucleic Acids Res. 16, 265–277 (1988). CAS PubMed PubMed Central Google Scholar Nishimasu, H. et al. Crystal structure of Cas9 in complex with guide RNA and target DNA. Cell 156, ...