Therefore, DNA isolation methods need to be adjusted according to the nature of metabolites. Several protocols have been developed for obtaining the best quality and highest yield of DNA from plants containing high levels of secondary metabolites. Some plants (e.g., mangrove and salt marsh) are ...
Hawkins TL, O'Connor-Morin T, Roy A et al. (1994) DNA purification and isolation using a solid-phase.Nucleic Acids Res22(21):4543-4544. Thermo Fisher Scientific, Inc. (2018) Qubit dsDNA assay specificity in the presence o...
To find anticancer agents from higher plants, DNA strand-scission assay method was employed for bioassay-guided fractionation as well as for screening the crude extracts. During the screening, an ethyl acetate extracts of the heartwood of Caesalpinia sappan L. (Leguminosae) exhibited potent DNA ...
plants, and bacteria species. For these organisms, it is recommended to include an extra step for cell lysis before disrupting the plasma membrane. This step is usually an enzymatic or mechanical process to disrupt the cell wall. The purification of DNA and RNA molecules is required to avoid ...
approach. Individual cells may be isolated frommicrobial communitiesusing methods like fluorescence-activated cell sorting (FACS) or microfluidic chips, which is then followed bycell lysisand isolation ofgenomic DNA(Lasken, 2012). Single-cell genomics was successfully used to sequence the genome from ...
DNA methylation is an evolutionarily conserved epigenetic mechanism essential for transposon silencing and heterochromatin assembly. In plants, DNA methylation widely occurs in the CG, CHG, and CHH (H = A, C, or T) contexts, with the maintenance of
DNA isolation. DNA for PCR analysis was isolated as previously described34, with minor modifications. Briefly, fresh P. patens tissue (approximately 10–20 mg) was collected in 1.5 ml microfuge tubes, frozen in liquid nitrogen, then ground using a plastic pestle. 400 μ L of ...
Genetic relationships amongGlycyrrhiza plants determined by RAPD and RFLP analyses. Biol Pharm Bull 1994; 17:1529. PubMed CAS Google Scholar Ho ISH, Leung FC. Isolation of DNA fingerprinting probe from Panax ginseng. Abstracts of Symposium on Chinese Medicine and Public Health, Hong Kong: ...
using an Easy Yeast Plasmid Isolation Kit (PT4073–1, Clontech), and was transformed into DH5αEscherichia coli. The recombined pGADT7-Rec2-ERF2 vector was isolated fromE. ColiusingE.Z.N.A.TM Plasmid Mini Kit (OMEGA). Screening the random DNA insertion library...
The viral DNA can, in principle, be recovered from the host cell at various times after infection for analysis at different stages of processing of the damage. There are, however, a few limitations associated with the use of viruses as probes. The viral DNA must be subject to repair by ...