[3] Tedesco S, De Majo F, Kim J, Trenti A, Trevisi L, Fadini GP, Bolego C, Zandstra PW, Cignarella A, Vitiello L. Convenience versus Biological Significance: Are PMA-Differentiated THP-1 Cells a Reliable Substitute for Blood-Derived Macrophages When Studying in Vitro Polarization? Front...
Methods THP-1 cells which differentiated into macrophages induced by phorbol ester (PMA),IFN-酌 were added to induce the activation of macrophages for twice. ELISA was used to detect the concentration of TNF-琢, IL-10, IL-12 andIL-23 in cultured supernatant in different stimulation ...
巨噬细胞表面与抗原提呈功能相关的CD分子的表达均较低,大部分呈阴性;而M1和M2细胞表面这些CD分子的表达均较高.结论单独使用PMA刺激THP-1细胞所分化而来的巨噬细胞抗原提呈能力较弱,基本处于静息状态.%Objective To identify the characteristics of the subtype of PMA-induced THP-1 macrophages by flow cytometry ...
Results THP-1 cells were differentiated into macrophages after the addition of PMA for 48h. P. g-LPS down-regulated the mRNA and protein ex-pression of CD36 as concentration increased and time extended in the presence of LDL (P < 0. 05). However,P. g-LPS had no effect on the ...
In conclusion,THP-1 cells could be differentiated into macrophages with PMA at concentrations of 1-100 ng/ml, and increasinginduction concentration could increased inflammatory factors secretion including TNF-α and IL-6, but had nosignificant effects on expression and distribution of membrane surface ...
Based on literature and on our experience[24–26], it can be summarized that PMA is the most effective differentiation agent to obtain mature THP-1 monocyte-derived macrophage with similarities to PBMC monocyte-derived macrophages. THP-1 monocytes can be fully differentiated into macrophages after ...
In THP-1 cells differentiated into macrophages, the H + current density was reduced by one-half, and τ act was slower by about twofold. The properties of H + channels in THP-1 cells and in other macrophage-related cells are compared....
THP-1 cells (cat # TIB-202, ATCC, Manassas, VA) were cultured in RPMI-1640 medium supplemented with 10% heat inactivated fetal bovine serum, 2 mMl-glutamine, 1 mM sodium pyruvate and 10 mM HEPES (cat#15630080, Life Technologies, Carlsbad, CA). THP-1 cells were differentiated into macrop...
比较不同诱导条件下的细胞THP-1细胞
THP-1 cells were differentiated into macrophages by culturing with 150 nM PMA for 2 days. 526 Exp. Mol. Med. Vol. 39(4), 524-534, 2007 Bioassay of TNF-α The TNF-α protein concentration was calculated using a TNF-α-sensitive mouse fibroblast cell line, L929. Briefly, L929 cells ...