New telomerase-immortalized cell line (A), produced from a human endometrial cell, isolated from uterine tissue in the proliferation phase of the cycle, and transfected with the eukaryotic expression plasmid pCI-neo-hTERT (= human telomerase reverse transcriptase) or pcDNA3-hTERTn2. Stable transfect...
【关键词】成釉细胞瘤;转染;永生化 Characterizationofgrowthandproliferationin a telomera.鸵-immortalizedameloblastomacelllineTAO Qian‘,埔Biao,QIAO Bin.zHENG Chao—qun,CHEN zK:fe峙‘Departmentof Oral and MaxillofacialSwge∥,Guanghua SchoolandHospital ofStomawlogy&InstituteofStomatofogical Research,Sun...
Telomeraseis not normally expressed in cells. The presence of telomerase activity may indicate the ability of a cell to bypass the telomeric “clock” that limits the proliferative capacity of normal somatic cells and may reflect the presence of immortalized or cancer cells. Measurements of telomerase...
All telomerase-negative immortalized cell lines examined to date show evidence of ALT activity, consistent with the hypothesis that telomere maintenance either by telomerase or by ALT is required for immortalization. The nature of the ALT mechanism(s) is currently unknown, but studies of telomere ...
Disrup- tion of BRCA1 function results in telomere lengthening and in- creased anaphase bridge formation in immortalized cell lines. Genes Chromosomes Cancer 2006 Mar;45(3):277-289. 70. Li B, Reddy S, Comai L. Depletion of Ku70/80 reduces the levels of extrachromosomal ...
It has been demonstrated that malignant transformation occurs when the telomerase in normal cell is activated [1, 2]. Telomerase activity is found in almost all malignant tumors [3]. Human telomerase RNA (hTR) is associated with the activity of telomerase, immortalized cancer cells retain the ...
Telomere dynamics in an immortal human cell line. The integration of transfected plasmid DNA at the telomere of chromosome 13 in an immortalized simian virus 40-transformed human cell line provided the fir... JP Murnane,L Sabatier,BA Marder,... - 《Embo Journal》 被引量: 710发表: 1994年 ...
[208] have found that TA possesses anti-proliferative properties, which was tested in vitro against seven human cancer cells and immortalized skin keratinocytes. Animal studies have also shown that regular dietary consumption of TA has strongly demonstrated dose-dependent chemopreventive actions against ...
[145]. Similarly, aTERTpromoter variant enhances EMT in prostate cancer and promotes the development of castration-resistant prostate cancer [140]. In HPV-immortalized cervical cancer cells, overexpression of TERT alters EMT characteristics [146]. The authors showed that TERT is a critical factor ...
It has been demonstrated that malignant transformation occurs when the telomerase in normal cell is activated [1, 2]. Telomerase activity is found in almost all malignant tumors [3]. Human telomerase RNA (hTR) is associated with the activity of telomerase, immortalized cancer cells retain the ...