New telomerase-immortalized cell line (A), produced from a human endometrial cell, isolated from uterine tissue in the proliferation phase of the cycle, and transfected with the eukaryotic expression plasmid pCI-neo-hTERT (= human telomerase reverse transcriptase) or pcDNA3-hTERTn2. Stable transfect...
The hepatocyte cell line is cloned and is immortalized by increasing telomerase activity in the cell. The hepatocyt... T Deurholt,R Hoekstra,RAF Chalumeau 被引量: 4发表: 2007年 Establishment of Immortalized Human Hepatocytes for Treatment of Liver Failure For practical use of bio-artificial ...
Disrup- tion of BRCA1 function results in telomere lengthening and in- creased anaphase bridge formation in immortalized cell lines. Genes Chromosomes Cancer 2006 Mar;45(3):277-289. 70. Li B, Reddy S, Comai L. Depletion of Ku70/80 reduces the levels of extrachromosomal te...
Mol. Cell. Biol. 22, 4512?4521 (2002). Article CAS PubMed Google Scholar Tokutake, Y. et al. Extra-chromosome telomere repeat DNA in telomerase-negative immortalized cell lines. Biochem. Biophys. Res. Commun. 247, 765?772 (1998). Article CAS PubMed Google Scholar Ogino, H. et al...
The present invention discloses a telomerase immortalized bovine thyroid cell lines. 本发明的牛甲状腺细胞系被命名为hTERT-BTY,并于2014年6月19日在中国典型培养物保藏中心(CCTCC)进行保藏,保藏号为C2014109. The invention of bovine thyroid cell line was named hTERT-BTY, and in June 19, 2014 were ...
thereby increasing the likelihood of tetraploidy and exacerbating chromosomal instability. At this juncture, activation of telomerase bypasses the restrictions imposed by aging and cell death, enabling the accumulation of mutations within the immortalized cell, which eventually evolves into malignant tumor c...
Soon after its discovery in Tetrahymena, telomerase activity was detected in other ciliated protozoa 13., 14., as well as in HeLa cells, an immortalized human cell line [15]. However, despite the efforts of several laboratories, the standard primer-extension assay was unable to detect telomerase...
However, most cell lines used in our study were established from tumor material and were not experimentally immortalized. In general, based on results of previous studies it cannot be assumed that all immortal cell lines have reacti- vated telomerase (33, 39, 40). We are unaware of investig...
These immortalized cells demonstrate the presence of fully differentiated odontoblast-specific markers, namely DMP1, DMP2, and DMP3, and also have the potential to produce a mineralized dentin matrix both in vitro and in vivo. The availability of this cell line will provide a unique opportunity ...
PDCD4, the protein encoded by the tumor suppressor gene PDCD4 (programmed cell death 4) has been implicated in the control of cellular transcription and translation by modulating the activity of specific transcription factors and suppressing the translat