Yingzi Hou et al, TDP-43 chronic deficiency leads to dysregulation of transposable elements and gene expression by affecting R-loop and 5hmC crosstalk, Cell Reports (2024). DOI: 10.1016/j.celrep.2023.113662
向溶液中添加1微升核糖核酸酶A(RNase A)(10 mg/ml)(Nacalai Tesque),并在37°C下培养1小时。通过加入等量的苯酚/氯仿/异戊醇(25:24:1)(Nippon Gene)和等体积的异丙醇(FUJIFILM Wako)沉淀基因组DNA,提取两次基因组DNA。17700离心g在4°C下放置12分钟,然后去除上清液并用70%的冰镇乙醇(FUJIFILM Wako)洗涤DNA...
2022年2月23日，美国斯坦福大学Aaron D. Gitler研究组以及美国梅奥医学中心Leonard Petrucelli研究组合作在Nature上发表了文章TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A；同期，英国伦敦大学学院皇后广场神经研究所Pietro Fratta研究组以及美国国立卫生研究院神经疾病和中风研究所Michael E. Ward...
2022年2月23日,美国斯坦福大学Aaron D. Gitler研究组以及美国梅奥医学中心Leonard Petrucelli研究组合作在Nature上发表了文章TDP-43 represses cryptic exon inclusion in the FTD-ALS gene UNC13A;同期,英国伦敦大学学院皇后广场神经研究所Pietro Fratta研究组以及美国国立卫生研究院神经疾病和中风研究所Michael E. Ward研...
[9] ALS News Today. (n.d.). ALS Gene Therapy SOL-257 Targeting TDP-43 Shows Promise in Mouse Model. Retrieved fromhttps://alsnewstoday.com/news/als-gene-therapy-sol-257-targeting-tdp-43-shows-promise-mouse-model/ [10] ALS News Today. (n.d.). Vectory Raises Millions to Advance AL...
Mutations in the TDP-43 gene and mislocalization of TDP-43 protein have been implicated in a growing number of neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Here, we show that TDP-43 negatively regulates innate immune gene ...
15.Ma, X.R., et al., TDP-43 represses cryptic exon inclusion in the FTD–ALS gene UNC13A. Nature, 2022: p. 1-7. 作者简介 任永飞、倪江霞,中国科学院上海有机化学研究所生物与化学交叉研究中心博士研究生。 陈椰林...
[9] ALS News Today. (n.d.). ALS Gene Therapy SOL-257 Targeting TDP-43 Shows Promise in Mouse Model. Retrieved fromhttps://alsnewstoday.com/news/als-gene-therapy-sol-257-targeting-tdp-43-shows-promise-mouse-model/ [10] ALS News Today. (n.d.). Vectory Raises Millions to Advance AL...
TDP-43 protein, encoded by theTARDBPgene, plays an important role in regulation of several processes, including microRNA processing, apoptosis, cell division, transcription, translation, splicing, axonal transport and neurite outgrowth1,2. Its major distinguishing features are the ability to bind RNA...
(miRNAs) and proteins, leading to increased levels of some proteins while functionally depleting others. Many of those functionally perturbed gene products are nuclear-genome-encoded mitochondrial proteins, and their dysregulation causes a global mitochondrial imbalance that augments oxidative stress. We ...