向溶液中添加1微升核糖核酸酶A(RNase A)(10 mg/ml)(Nacalai Tesque),并在37°C下培养1小时。通过加入等量的苯酚/氯仿/异戊醇(25:24:1)(Nippon Gene)和等体积的异丙醇(FUJIFILM Wako)沉淀基因组DNA,提取两次基因组DNA。17700离心g在4°C下放置12分钟,然后去除上清液并用70%的冰镇乙醇(FUJIFILM Wako)洗涤DNA...
Fig. S10: IRES-TARDBP gene knocked into the stop exon of the Thy1 gene via CRISPR/Cas9 strategy. Supplementary Table 1: Primers used to identify the genotype of Thy1-e(IRES-TARDBP) 1 mice. Supplementary Table 2: Antibodies used in the study....
There is a need to define the presence of the major ALS mutations, including the C9orf72 expansion mutations, SOD1 and TARDBP gene mutations, in MMC residents, and this study further highlights the need to consider early TDP-43 pathological changes as an opportunity to explore pathomechanistic ...
TDP-43 has multiple functions in gene transcription and translations3,16. RRMs of TDP-43 bind to both single- and double-stranded TG-DNAs and UG-rich RNA17, and HIV trans-activation response element (TAR) DNA18. TDP-43 dimerization has been shown by size- exclusion chromatography (SEC) ...
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