向溶液中添加1微升核糖核酸酶A(RNase A)(10 mg/ml)(Nacalai Tesque),并在37°C下培养1小时。通过加入等量的苯酚/氯仿/异戊醇(25:24:1)(Nippon Gene)和等体积的异丙醇(FUJIFILM Wako)沉淀基因组DNA,提取两次基因组DNA。17700离心g在4°C下放置12分钟,然后去除上清液并用70%的冰镇乙醇(FUJIFILM Wako)洗涤DNA...
Fig. S10: IRES-TARDBP gene knocked into the stop exon of the Thy1 gene via CRISPR/Cas9 strategy. Supplementary Table 1: Primers used to identify the genotype of Thy1-e(IRES-TARDBP) 1 mice. Supplementary Table 2: Antibodies used in the study....
Transactive response DNA binding protein 43 kDa (TDP-43) was discovered in 2001 as a cellular factor capable to inhibit HIV-1 gene expression. Successively, it was brought to new life as the most prevalent RNA-binding protein involved in several neurological disorders, such as amyotrophic lateral...