摘要:【目的】设计和构建针对STAT3基因的siRNA表达框架,并在结直肠癌细 胞中观察其干扰效果.【方 法】GeneBank中查询STAT3基因序列,Oligo6.0软件设计siRNA靶序列,合成 并修饰靶序列后PCR扩增siRNA表 达框架,通过脂质体介导,直接将扩增产物转染至结直肠癌细胞SW480中,48h后 提取细胞总RNA进行RT— PCR检测干扰效果.【...
Methods:Design and synthetise three siRNA sequences targeting STAT3,then select one with best gene interference effect,and construct STAT3-shRNA vector,pSUPERretro-puro-STAT3. The recombinant plasmid was transfected into packaging cell line 293FT and then infected into HPV16 positive cervical cancer ...
CpG-STAT3 siRNA, is a tumor vaccine that co-delivers CpG and STAT3 siRNA oligonucleotide, achieving a whole-body immune response and inhibiting the immune suppressive environment in TME [296]. Since STAT3 has well-described roles in myeloid cells and Treg in promoting the immunosuppressive effects...
3f). Subsequently, we transfected M2-like macrophages with a STAT3 overexpression vector (STAT3-OE) or STAT3 siRNA (si-STAT3) to overexpress or knock down STAT3, respectively (Fig. S5a). After validating the overexpression and knockdown effects, we found that the change in IL-6 expression ...
b A volcano plot of RNA-seq data from Norad KD versus control siRNA in MEF cells at 24 h post-transfection. Differentially expressed genes (Padj < 0.05, -2 > FC > 2) are in bold, ISGs are marked in red (n = 3). c The FCs of differentially expressed genes ...
产品活性:Colivelin 是一种具有大脑通透性的神经保护肽 (neuroprotective peptide),是 STAT3 的有效激活剂。Coliveli 通过激活 STAT3 在体外抑制神经元死亡。Colivelin 对神经毒性、Aβ 沉积,神经元细胞凋亡,在神经退行性疾病显示出长期有利的影响。Colivelin 具有潜力用于阿尔茨海默病和缺血性脑损伤的相关研究。 体...
The transfected cells were cultured at 37 °C for 48 h, and then the efficiency of siRNA transfection was assessed by western blot analysis. The siRNA sequences are listed as follows: STAT3 siRNA1: 5’-CCACGTTGGTGTTTCATAA-3’; STAT3 siRNA2: 5’-GCAGGATCTAGAACAGAAA-3’; STAT3 siRNA3...
Scrambled siRNA (Scr) was used as a negative control with the sequence: 5ʹ-UUCUCCGAACGUGUCACGUTT-3ʹ. The siRNAs were purchased commercially (Gene Pharma Inc., Shanghai, China). Chordoma cells were transfected with siRNA at a final concentration of 50 nM using Lipofectamine 3000 ...
1成功体外合成stat3基因的siRNA表达载体。 2 STAT3.siRNA表达载体转染SMMC.7721后,stat3基因 表达下调,并下调了pim.2基因表达。 3 RNA干扰(RNAinterference,RNAi)沉默stat3的治疗策略可 能为肝癌治疗提供新的靶点。 体 关键词:肝癌;pim一2;RNA干扰;siRNA;表达载 ...
sh/siRNA sequences, see Table S3 this paper N/A ChIP-PCR primers, see Table S5 this paper N/A RT-qPCR primers, see Table S6 this paper N/A Recombinant DNA CHI3L1-Flag this paper N/A CHI3L1-GST this paper N/A STAT3-HA this paper N/A CHI3L1 WT and mutants subcloned into the ...