When added to three unique negative control siRNA sequences, which should not target any transcript, the Silencer Select modifications reduced the number of differentially expressed genes, compared to mock-transfected cells by an average of 83% (Figure 5). When t...
Negative Control Kit contains all three controls (Lo, Med, Hi GC content-excluding sequences #2 and #3) and each is also available separately. Get the ideal negative controls for your RNAi experiment so you can assess your results accurately. 仅供科研使用。不可用于诊断程序。 规格 适用于(...
If not, we will synthesize 3 new siRNA sequences for free. Pre-designed siRNAs Set A Products Quantity Purification Target Gene siRNA Oligos 3 candidates (1×2 OD) HPLC Negative Control siRNA Oligos 1 OD HPLC FAM-labeled Negative Control siRNA Oligos 1 OD HPLC Positive Control siRNA ...
A negative control siRNA with the same nucleotide composition as your siRNA but which lacks significant sequence homology to the genome. To design a negative control siRNA, scramble the nucleotide sequence of the gene-specific siRNA and conduct a search to make sure it lacks homology to any other...
Positive control: A known siRNA to give a high knockdown of the target of interest. Negative control: A non-silencing siRNA helps to identify non-specific changes in gene expression. Another siRNA against the same target: A second siRNA against the same target but against another region of the...
原文引用:The siIDO1 (sense strand 5′-GCAAUAGUAGAUACUUACA-3′), the negative control siRNA (a scrambled sequence consisting of the sense strand 5′-UUCUCCGAACGUGUCACGUTT-3′) and the Cy3-siRNA were synthesized by Genomeditech Biotechnology Co., Ltd ...
Control Type:Negative Control RNAi Type:siRNA Shipping Condition:Dry Ice Product Line:Silencer™, Ambion™ 储存 Store at or below -20°C. Do not store in a frost-free freezer. 描述 Ambion®Silencer®Negative Control #5 siRNA has no significant sequence similarity to mouse, rat, or huma...
sequence homology between this siRNA and all known human genes.The results indicated that this siRNA cannot be used as negative control in erythroid differentiation experiments.The effect of siRNA was far more complicated than it was expected.Investigators should pay much attention to the off-target ...
Always design negative controls by scrambling targeted siRNA sequence. The control RNA should have the same length and nucleotide composition as the siRNA but have at least 4-5 bases mismatched to the siRNA. Make sure the scrambling will not create new homology to other genes. ...
Ambion® Silencer Negative Control #1 siRNA, In Vivo Ready has no significant sequence similarity to mouse, rat, or human gene sequences. The control has also been tested in cell-based screens and proven to have no significant effect on cell proliferation, viability, or morphology. It is hig...