How to Tackle the Challenge of siRNA Delivery with Sequence-Defined Oligoamino Amidesdoi:10.1002/mabi.201600152Sren ReinhardErnst WagnerJohn Wiley & Sons, LtdReinhard, S., and Wagner, E. (2017) How to Tackle the Challenge of siRNA Delivery with Sequence-Defined Oligoamino Amides. Macromol. Bio...
Sequence-independent self-assembly of germ granule mRNAs into homotypic clusters. Mol. Cell 78, 941–950.e12 (2020). This study reveals that germ granules organize their mRNAs into distinct clusters through a self-sorting mechanism inherent to the mRNA. Article CAS PubMed PubMed Central Google ...
aThe cognate sequence of an siRNA is either located on the untranslated 5′region of an mRNA, the open reading frame, or the 3′end of the message. siRNA的同源序列位于mRNA、开放读书框架或者消息的3 ′末端的未翻译的5 ′区域。[translate] ...
Obviously their genomes would be flexible enough to include short late domain sequences to recruit ESCRTs. In the absence of ESCRTs, one or several factors, viral or yet unknown cellular factors, may set the stage for membrane fission. In case of influenza virus, the mechanism of hydrophobic...
a siRNA drug that treats hereditary transthyretin-mediated amyloidosis, a neurodegenerative disease caused by a defective gene. Patisiran is encapsulated in an LNP to protect it until it reaches the liver, where it then prevents expression of the defective gene. Patisiran was approved in the United...
aFor design siRNA targeting u-PA, a DNA sequence of the type AA(N19) was selected. 正在翻译,请等待...[translate] acoverstitch coverstitch[translate] aI can manage it myself. 我可以处理它我自己。[translate] aThe initiative for a long time will be very tired, care about for a long ti...
A. What does it mean to 'knock-out' a gene? B. How would the process of knocking out a gene aid in determining a function of the gene? The Roles of DNAs and Proteins: Genes are stretches of DNA that have specific seq...
In light of these, NP-based siRNA delivery systems can be a promising approach to silence the SARS-CoV-2 genome targets to halt the infection. 2.1.3. Neutralizing antibodies (nAbs) In addition to what has been mentioned, the other important target for developing antivirals against SARS-CoV-...
to safer pharmaceutical properties, such as no risk of genome integration, the transient expression of mRNA-encoded antibodies enables a more controlled exposure, with more protein production during peak expression compared to naked pDNA [17]. Because additional sequences such as plasmid backbone are...
(H) Real-time PCR quantification of mRNAs in MCF-7 cells in the presence of solvent control or 5 μM of BAY 11–7082 left untreated or exposed to LzCD95L for 4 days. (I) Western blot analysis of MCF-7 cells transfected with control siRNA (siCtr) or smart pool siRNA against IRF3 ...