Each 3' end of the duplex is protected from polyuridylation and degradation by a methyl group added by the methyltransferase HUA ENHANCER 1 (HEN1). The miRNA duplex is shuttled to the cytoplasm by HASTY (HST). In there, the steady-state level of miRNAs is controlled by the exonuclease ...
- 《Cancer Biology & Therapy》 被引量: 134发表: 2006年 Long-term suppression of telomerase expression in HeLa cell clones, transfected with an expression vector carrying siRNA targeting hTERT mRNA. HeLa cell cultures were used as model systems for small interfering RNA (siRNA) induced knockdown ...
摘要 Objective To investigate the relationship between the level of Bcl-2 in human hepatocellular carcinoma( HCC)tissues a... 被引量 16 收藏 全部来源 求助全文 Semantic Scholar 知网 NCBI NCBI Europe PMC 查...
ObjectivePrimary human preadipocytes and differentiated adipocytes in culture are valuable cell culture systems to study adipogenesis and adipose function in relation to human adipose biology. To use these systems for mechanistic studies, siRNA-mediated knockdown of genes for its effectiveness was studied....
Mammalian sex determination: A molecular drama A biweekly scientific journal publishing high-quality research in molecular biology and genetics, cancer biology, biochemistry, and related fields A Swain,R Lovell-Badge - 《Genes & Development》 被引量: 618发表: 1999年 加载更多来源...
关键词:A型流感病毒,RNA干扰,shRNA,姒Ⅱ中山大学硕士论文谢征siI冰A干扰A型流感病毒初步研究PrimaryresearchoninterferenceofInfluenzaAvirusbyshortinterferingRNAMajor:BiochemistryandMolecularBiologyName:ZhengXieSupervisor:Prof.AnlongXuAbstractInfluenzaAvirus,whichbelongstoOrthomyxoviridae,Influenzavirusgenus,couldcausethe...
Hybrid modalities, especially RNA conjugates, continue to enable scientists to pursue undruggable targets. Building on Benchling's previous releases for macromolecules at the intersection of chemistry and biology, comes the first in a series of updates allowing you to model oligonucleotide-based entities...
depend on partial complementarity for mRNA binding, while siRNA rely on 100% complementarity [6]. It is worth noting that a single siRNA or miRNA can bind to several mRNA targets, but their intracellular enzymatic activity eventually destroys them after dilution beyond the therapeutic level [11]....
Shown are structures of sugar, backbone and base modifications and of the cholesterol conjugate.Natue Chemical Biology, 2 (2006), 711-719.Nature reviews drug discovery, 6 (2007), 443-453高分子纳米载体高分子纳米载体抗体类抗体类化学修饰化学修饰siRNAsiRNA缀合物缀合物Potentia 10、l of siRNA-Based...
Each 3' end of the duplex is protected from polyuridylation and degradation by a methyl group added by the methyltransferase HUA ENHANCER 1 (HEN1). The miRNA duplex is shuttled to the cytoplasm by HASTY (HST). In there, the steady-state level of miRNAs is controlled by the exonuclease ...