Single-nuclei isolationsnRNA-seqArabidopsis thaliana seedlingsflower developmentantherSingle-cell genomics provides unprecedented potential for research on plant development and environmental responses. Here, we introduce a generic procedure for plant nuclei isolation combined with nanowell-based library ...
Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder the study of isoforms. Here we present single-nuclei isoform RNA sequencing (SnISOr-Seq). Using microfluidics...
Single nucleus RNA sequencing (snRNA﹕eq) has the potential to improve our understanding of the cellular﹕pecific drivers of sporadic Alzheimer's disease. In this study we re゛nalyze published snRNA﹕eq data from post﹎ortem dorsolateral prefrontal cortex tissue from 48 participants in the ...
Single-nuclei RNA Profiling Reveals Disruption of Adipokine and Inflammatory Signaling in Adipose Tissue of Burn Patients Moreover, using single-nuclei RNA sequencing, we provide the first comprehensive characterization of alterations in the adipose tissue microenvironment occurring ... K Carly M.,R Zach...
this technology is constrained to the analyses of fresh tissue. Single-nuclei RNA sequencing (snuclRNA-seq) is still in its initial developmental stages. In this study, we showed that it is feasible to employ it to ascertain highly informative and unique brain tissue collected during many years...
(n = 6), one from the macula region and the other from the peripheral region, were collected and subjected to single-cell nuclei RNA-Seq. After dispensing, nanowells were imaged and only wells with single nuclei were selected. cDNA library construction and sequencing were performed for a...
Single nuclei RNA sequencing (snRNA-seq) has evolved as a powerful tool to study complex human diseases. Single cell resolution enables the study of novel cell types, biological processes, cell trajectories, and cell–cell signaling pathways. snRNA-seq largely relies on the dissociation of intact ...
sequencingthetranscriptomeofacellnucleus.Nucleiareisolated fromspecimensandsortedbyFACS,cDNAlibrariesareconstructedandRNA-seqisperformed, followedbydataanalysis.Somestepsfollowpublishedmethods(Smart-seq2forcDNAsynthesis andNexteraXTbarcodedlibrarypreparation)andarenotdescribedindetailhere.Previoussingle- cellapproaches...
Bulk brain tissue cell-type deconvolution with bias correction for single-nuclei RNA sequencing data using DeTREM O’Neill NK, Stein TD, Hu J, Rehman H, Campbell JD, Yajima M, Zhang X, Farrer LA BMC Bioinformatics. 2023 Sep 19https://doi.org/10.1186/s12859-023-05476-w ...
Complete analysis workflow for single-cell/nuclei RNA-sequencing data. workflow pipeline nextflow rnaseq single-cell nf-core single-nuclei-rna-sequencing single-nuclei Updated Jun 30, 2023 Nextflow Cadaei-Yuvxvs / snRNAseq_muscle_MND Star 0 Code Issues Pull requests Single Nuclei RNA ...