这种策略有两个优势:(1)通过计数每种UMI的数目可以得到样本中TCR序列的原始分布,这有助于更精确的定量TCR克隆频率;(2)通过将具有相同UMI的reads聚集在一起可以有效矫正PCR和测序错误,从而可以推断出真实的TCR序列。由于TCRs序列之间的差异仅有几个核苷酸,这种矫正步骤可以区分真实的TCR变异和错误导致的假的多样性,所...
懿贝瑞:Single-Cell RNA-Seq助力肿瘤突变抗原特异性TCRs的发现众所周知,T-cell receptors(TCR)的作用是识别抗原,在肿瘤免疫治疗当中发挥着重要作用。TCR分为两类:TCR1和TCR2,TCR1由γ和δ两条链组成,TCR2…
使用Chromium GEM-X单细胞免疫分析(5 ')v3,该检测的一个关键特征是对配对B细胞或T细胞受体(BCR或TCR)的全长V(D)J序列的免疫细胞库进行全面分析。平均V-J配对率为95%,而10X单细胞免疫图谱(5 ')v2的配对率为84%。研究小组还观察到T细胞受体基因TRA和TRB的检测表达分别增加了200%和120%。 增强BCR和TCR的表征...
Clonal expansions occur in the persistent HIV reservoir as shown by the duplication of proviral integration sites. However, the source of the proliferation of HIV-infected cells remains unclear. Here, we analyze the TCR repertoire of single HIV-infected
2b), and the bulk of reconstructed TCR sequences corresponded to expected single-chain pairing (Fig. 2c). Consistently higher gene detection was observed across cell types for Smart-seq3xpress in comparison to Smart-seq2 (ref. 15) and Smart-seq3 (ref. 2) (Fig. 2d). A recent study14...
Single-cell sequencing technologies for CAR T-cell therapy.(A)Schematic overview of single-cell multi-omics analysis of CAR T-cell therapy.(B)scRNA-seq measures the transcriptome from the entire cell.(C)scTCR-seq measures the V(D)J sequence of TCR and gene expression profiles in tandem.(D...
We present TRAPeS (TCR Reconstruction Algorithm for Paired-End Single-cell), a software for reconstruction of T cell receptors (TCR) using short, paired-end single-cell RNA-sequencing. TRAPeS reconstruct the TCR in 3 steps: For each chain, it first identify the V and J segments by searchin...
2P Single-cell profiling and integrative TCR analysis reveals tumor-mutation associated phenotypes and immune repertoire in lung adenocarcinomadoi:10.1016/j.annonc.2024.08.012A.X. LozanoM. GhaediA. AzarminaA. SayadS.C. LienS. SoleimaniD.C. Chung...
Figure S6. Additional Details on Paired Single-Cell TCR Sequencing and RNA-Seq of T Cells from Three Breast Tumors, Related toFigure 6 (A) Clinical and related metadata for 3 tumors (BC9-11) used for paired TCR and RNA-seq study. ...
Moreover, patients with low TRLS also showed higher TCR richness and Shannon entropy of TCR diversity, whereas patients with high TRLS were remarkably featured by high levels of single-nucleotide variant (SNV) neoantigens, indel neoantigens, cancer-testis antigens (CTA) score, intratumor ...