TCR介导的抗原识别依赖于TCR与抗原主要组织相容性复合体（MHC）分子的相互作用。每个人的TCR库高度多样化，这种多样性有利于识别多种外源抗原，但同时也对传统鉴定TCR库方法提出了挑战。高通量测序技术的引入，使得研究者能够大规模分析TCR序列，从而促进了TCR库研究的进展。高通量测序技术使得TCR库研究成为可...
为了克服上述挑战，上海懿贝瑞提出了利用单细胞RNA测序（Single-Cell RNA-Seq）技术来发现肿瘤突变抗原特异性TCRs的新方法。单细胞测序能够揭示每个细胞的独特变化，甚至揭示全新的细胞类型，极大地推进了基因组学领域的发展，使科学家们可以在单细胞水平上进行分子机制研究。这一方法通过分离患者TILs，与来源...
Single-cell full-length TCR/BCR assays provide the ability to profile the highly diverse T cell receptor (TCR) and B cell receptor (BCR) repertoires of the adaptive immune response. The identification of T cell clonotype can be challenging due to insufficient sensitivity to detect low abundance ...
就比如,在乳腺癌中,Single-Cell Seq揭示了三阴性乳腺癌患者受到化疗后,部分患者反而生存时间缩短,究其原因,是因为肿瘤异质性引起的耐药克隆的存在。 Kim C, Gao R, Sei E, et al. Cell, 2018, 173(4): 879-893. e13. 目前在神经胶质瘤、头颈部癌等、多种肿瘤研究中,单细胞测序都有广泛的应用,涉及肿瘤...
(n= 24 channels, 26,191 cells) is shown on the left with all major immune cell populations identified. Clustering and cell-type populations are highly concordant across chip channels and experimental weeks, underscoring the high inter- and intra-chip reproducibility of our microfluidic technology ...
. TCR sequences retrieved from distinct single sorted cells were compared. Clonotypes were defined either as expanded (i.e. detected in at least two cells) or unique (i.e. detected in no more than one cell). The memory phenotype of individual cells was also recorded during index cell ...
A hallmark of celiac disease (CeD), a chronic condition driven by cereal gluten exposure, is increase of gut intraepithelial γδ T cells. This may indicate pathogenic involvement of γδ T cells and existence of disease-specific γδ T-cell receptors (T
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-specific markers and cell types. scRNA-seq plays...
Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially
[20]. PD-1 is another immune checkpoint that binds to the ligands PD-L1 and PD-L2, inducing the suppression of T cell receptor (TCR)-mediated proliferation and the CD28-mediated costimulation pathway [21,22]. Inhibiting the interaction between PD-1 and PD-L1 can enhance the anti-tumor ...