Single-cellT cell receptor (TCR)10x Genomics is a highly accessible single cell RNA sequencing platform that allows for simultaneous gene expression analysis and identification of receptor chain combinations in cells of the adaptive immune system. Here, we asked whether the gene and receptor ...
Single cell sequencing allows scientists to see the unique gene expression patterns of each cell. This view more fully characterizes tissue heterogeneity, revealing the rare cell types that have big consequences in health and disease. Get to know the method changing how we see biology ...
Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially reduce reagent use and increase cellular throughpu...
10X Genomics运用微流控系统进行细胞分选，带有Barcode的Gel Beads 与水象的细胞和酶相结合，进入油相后...
Aran摘要Since the first publication a decade ago describing the use of single-cell RNA sequencing ...
来自单个细胞的所有cDNA,源自单个Gel Bead,将具有相同的10X条形码,能够将每个转录本映射回其起源细胞。然而,单个凝胶珠上的单个寡核苷酸具有不同的UMIs,这使得每个cDNA分子具有独特的随机12碱基序列。这允许在下游处理过程中消除任何PCR重复。 然后将条形码cDNA用于制备测序文库,其中包括sequencing adapters的添加和PCR扩增...
10x Genomics Single-Cell Sequencing Details Demo FAQ Case Study CD genomics is now offering single cell analysis utilizing various solutions of the 10X Genomics Chromium system, allowing the profiling of rare or heterogeneous populations of cells. The Introduction of 10x Sequencing Powerful approaches ...
In all, 1/22.5 of total cDNA was used to generate single-cell TCR sequencing libraries. Single-cell TCRγδ sequencing libraries were generated using the Chromium Single Cell 5′ Library construction kit (PN1000020, 10X Genomics) and custom primer sets as below. 1st PCR: 2 μM of ...
Dual-Indexed Sequencing Run: Single Cell 5' v3 libraries are dual-indexed. We do not recommend sequencing 10x Single Cell 5’ v3 libraries with a single-index configuration. See:Can single index and dual index libraries be pooled for sequencing?
Thus, although bold changes in γδ T-cell repertoires occur as a consequence of CeD, whether this is a primary or secondary phenomenon in the disease remain unclear. Results Establishment of a single-cell paired γδ TCR sequencing platform In order to characterize the γδ TCR repertoire ...