Here, we extend the application of the HIVE by offering a single-cell T cell receptor enrichment and sequencing (scTCRseq) assay. Using the stable whole transcriptome amplification (WTA) product obtained from the scRNAseq assay as the sample input, the HIVE scTCRseq assay enriches TCR in a ...
8012了,高通量测序早已非常普遍,走在生命科学前沿的研究者们几乎都在谈论Single-Cell Sequencing对于整个生命科学研究的意义以及价值。无论是肿瘤、发育、神经科学,还是血液免疫或者其他领域,生物学研究似乎进入了一个新的时代——单细胞时代(Single Cell Era),我们的视野越来越精准,从一块组织转为一块组织中的每一个...
Single-cell RNA sequencing (scRNA-seq) can be used to identify and characterize distinct immune cell subsets in health and disease. The transcriptional signatures of these immune cells enable the identification of novel pathogenic drivers and biomarkers. scRNA-seq can be used to identify stochastic ...
is increase of gut intraepithelial γδ T cells. This may indicate pathogenic involvement of γδ T cells and existence of disease-specific γδ T-cell receptors (TCRs) recognizing defined antigen(s). We performed high-throughput and paired γδ TCR sequencing of single intraepithelial γδ...
(Daver et al.2019). Two studies utilized single-cell TCR sequencing, immunological profiling, scRNA-seq, and CITE-seq on AML patients treated with a combination of hypomethylating drugs and PD-1 inhibitors to explore the causes of resistance to ICI (Goswami et al.2022; Abbas et al.2021)...
Use of scRNA-seq can capture the changes among different CD8+T cell subsets during ICB. Integration of TCR sequencing (TCR-seq) with scRNA-seq provides insights into CD8+T cell clonality, diversity, and dynamics in both human and animal models [98]. ...
题目:Single-cell RNA sequencing ofperipheral blood mononuclear cells from acute Kawasaki disease patients 时间:2021年9月 期刊:Nature communications (IF: 12.298) 链接:https://doi.org/10.1016/j.immuni.2020.07.009 作者利用单细胞RNA以及BCR/TCR测序对急性KD患者PBMCs进行分析,通过静脉注射免疫球蛋白(IVIG)...
TCR介导的抗原识别依赖于TCR与抗原主要组织相容性复合体（MHC）分子的相互作用。每个人的TCR库高度多样化，这种多样性有利于识别多种外源抗原，但同时也对传统鉴定TCR库方法提出了挑战。高通量测序技术的引入，使得研究者能够大规模分析TCR序列，从而促进了TCR库研究的进展。高通量测序技术使得TCR库研究成为...
Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially reduce reagent use and increase cellular throughpu...
cell receptor (TCR) sequencing data, TCR utilization in T-cell phenotypic diversity was discovered29. The differences in the TCR clonotype composition and key gene expression of individual T-cell clusters indicate that the phenotypic state may be formed by antigenic TCR stimulation and environmental ...