例如CellMarker（28），PangaloDB（29）和ImmuCellDB（30），它们利用通过文献手工核查确定的特定于细胞类...
T细胞受体(T cell receptor,TCR)是T细胞表面负责特异性识别与MHC(主要组织相容性复合体)结合的抗原肽的蛋白。当TCR与抗原肽和MHC结合时,T淋巴细胞通过信号转导被激活,启动免疫应答。 人类基因组中有4个TCR基因:两个编码轻链TCR:TRA基因编码TCRα,TRG基因编码TCRγ;两个编码重链TCR:TRB基因编码TCRβ,TRD基因编码TCR...
We performed high-throughput and paired γδ TCR sequencing of single intraepithelial γδ T cells of untreated CeD patients (=8; 1821 cells), CeD patients treated with a gluten-free diet (=5; 436 cells) and controls (=7; 1068 cells). We found that CeD patients, both untreated and ...
Thus, although bold changes in γδ T-cell repertoires occur as a consequence of CeD, whether this is a primary or secondary phenomenon in the disease remain unclear. Results Establishment of a single-cell paired γδ TCR sequencing platform In order to characterize the γδ TCR repertoire ...
cell sorting of HIV-infected (p24+) cells by HIV-Flow33with multiplex PCR of the V–J junction of the TCRβ chain (including CDR3 region) followed by sequencing (Supplementary Fig.1a). TCR sequences retrieved from distinct single sorted cells were compared. Clonotypes were defined either as...
Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing 这篇文章[1]的一个亮点在于对肿瘤中的T细胞有了认识。 data 不得不说,这个样本的数量和质量真的太好了,一共是六个病人的外周血,正常组织,癌症区域配对样本,形成了非常好的对照。不知道是不是因为P1202病人T细胞的数量太少...
ARTICLESingle-cell TCR sequencing of gut intraepithelial γδ T cellsreveals a vast and diverse repertoire in celiac diseaseLinn M. Eggesbø 1 , Louise F. Risnes 1,2 , Ralf S. Neumann 1 , Knut E. A. Lundin 1,3 , Asbjørn Christophersen 1 and Ludvig M. Sollid 1,2A hallmark of ...
Use of scRNA-seq can capture the changes among different CD8+T cell subsets during ICB. Integration of TCR sequencing (TCR-seq) with scRNA-seq provides insights into CD8+T cell clonality, diversity, and dynamics in both human and animal models [98]. ...
8012了,高通量测序早已非常普遍,走在生命科学前沿的研究者们几乎都在谈论Single-Cell Sequencing对于整个生命科学研究的意义以及价值。无论是肿瘤、发育、神经科学,还是血液免疫或者其他领域,生物学研究似乎进入了一个新的时代——单细胞时代(Single Cell Era),我们的视野越来越精准,从一块组织转为一块组织中的每一个...
A brief method and principle of single-cell multi-omics technologies for (A) scTCR/BCR-seq, (B) ClickTag, (C) proteome, (D) microbiome, (E) metabolome, (F) epigenome Full size image New advances in single-cell sequencing Although high-throughput single-cell sequencing has revealed the ge...