Bulk T cell repertoire sequencing (TCR-Seq) has emerged as a powerful method to profile the T cell repertoire of a sample in a high throughput fashion. Given the increasing utilization of TCR-Seq, we aimed here to provide a comprehensive, up-to-date review of the method, its extensions, ...
Clonal expansions occur in the persistent HIV reservoir as shown by the duplication of proviral integration sites. However, the source of the proliferation of HIV-infected cells remains unclear. Here, we analyze the TCR repertoire of single HIV-infected
We performed high-throughput and paired γδ TCR sequencing of single intraepithelial γδ T cells of untreated CeD patients (n = 8; 1821 cells), CeD patients treated with a gluten-free diet (n = 5; 436 cells) and controls (n = 7; 1068 cells). We found that CeD patien...
Next‐generation TCR sequencing–a tool to understand T‐cell infiltration in human cancersdoi:10.1002/path.4800Tumour-infiltrating lymphocytes (TILs) are ... I Poschke,M Flossdorf,R Offringa - 《Journal of Pathology》 被引量: 0发表: 2016年 T cell infiltration of the prostate induced by andro...
In conclusion, our single-cell sequencing study has revealed that untreated CeD patients have a higher degree of diversity in the repertoire from γδ TCR IELs and that these changes are also present in CeD patients on a gluten-free diet. Despite these CeD-specific findings, we are unable to...
allorecognition; alloreactivity; transplantation; direct pathway; indirect pathway; TCR sequencing; HTS1. Introduction The problem of allorecognition has been relevant since the establishment of the leading role of MHC (major histocompatibility complex) molecules in organ and tissue rejection. Understanding...
(s). We performed high-throughput and paired γδ TCR sequencing of single intraepithelial γδ T cells of untreatedCeD patients (n =8; 1821 cells), CeD patients treated with a gluten-free diet (n= 5; 436 cells) and controls (n=7; 1068 cells). Wefound that CeD patients, both ...
et al. Next generation sequencing for TCR repertoire profiling: platform-specific features and correction algorithms . European journal of immunology 42 , 3073–3083, (2012).10.1002/eji.201242517 [ Cross Ref ]Bolotin DA, Mamedov IZ, Britanova OV, Zvyagin IV, Shagin D, Ustyugova SV, et al....
We performed high-throughput and paired γδ TCR sequencing of single intraepithelial γδ T cells of untreated CeD patients (=8; 1821 cells), CeD patients treated with a gluten-free diet (=5; 436 cells) and controls (=7; 1068 cells). We found that CeD patients, both untreated and ...
Single-cell TCR sequencing of gut intraepithelial γδ T cells reveals a vast and diverse repertoire in celiac diseaseA hallmark of celiac disease (CeD), a chronic condition driven by cereal gluten exposure, is increase of gut ... LM Eggesb,LF Risnes,RS Neumann,... - 《Mucosal Immunology》...