[1] Zhang Y, Hu J, Ji K, et al. CD39 inhibition and VISTA blockade may overcome radiotherapy resistance by targeting exhausted CD8+ T cells and immunosuppressive myeloid cells[J]. Cell Reports Medicine, 2023, 4(8). [2] Gao S, Wu Z, Arnold B, et al. Single-cell RNA sequencing cou...
TCR测序哪家强,认准康测别瞎忙 TCR测序,也称为T细胞受体库测序(T cell receptor repertoire sequencing,TCR-Seq)靶向扩增T细胞受体的互补决定区(CDR区),并通过高通量测序技术分析其多样性。 正常个体在无抗原刺激时,TCR基因重排是随机的,因此正常人外周T细胞呈多家族、多...
Quantitative T cell repertoire analysis by deep cDNA sequencing of T cell receptor α and β chains using next-generation sequencing (NGS). OncoImmunology 2014. Highly Clonal Regulatory T cell population in Follicular Lymphoma – Inverse Co...
A series of studies have corroborated that T-cell Receptor (TCR) repertoire serves as a biomarker for monitoring immune responses. CD Genomics has pioneered the development of an advanced high-throughput sequencing method, TCR-Seq, to characterize this TCR library. ...
[9] Bai X, Zhang Q, Wu S, Zhang X, Wang M, He F, et al. Characteristics of tumor infiltrating lymphocyte and circulating lymphocyte repertoires in pancreatic cancer by the sequencing of T cell receptors. Sci Rep. 2015;5: 13664. doi:10.1038/srep13664. ...
作为2020年的最后一贴,今天来跟大家谈谈如何分析TCR-seq的数据,全称T-cell receptor sequencing。该方法诞生的目的就是用生物信息学手段来全面高速地分析高通量测序技术检测靶向扩增后的T细胞抗原识别决定性表面分子,即T细胞受体多样性的检测技术,用以揭示机体在生理和病理状态下T细胞介导的细胞免疫应答状态改变。如下面的...
High-throughput 3′ single-cell RNA-sequencing (scRNA-seq) allows cost-effective, detailed characterization of individual immune cells from tissues. Current techniques, however, are limited in their ability to elucidate essential immune cell features, including variable sequences of T cell antigen ...
[8] Pai J A, Satpathy A T. High-throughput and single-cell T cell receptor sequencing technologies[J]. Nature methods, 2021, 18(8): 881-892. [9] Jiang, X., et al., Comprehensive TCR repertoire analysis of CD4(+) T-cell subsets in rheumatoid arthritis. J Autoimmun, 2020. 109: p...
为了在单细胞水平实现深度的T细胞分析,Fluidigm在C1™系统上推出了免疫学应用–Single-Cell TCR Sequencing。这个应用可揭示每个细胞的T细胞受体(TCR)α和β链,同时获得相应的基因表达特征。 这种方法是由欧洲分子生物学实验室(EMBL-EBI)和Sanger...
标题:Multi-region sequencing unveils novel actionable targets and spatial heterogeneity in esophageal squamous cell carcinoma 多区域测序揭示了食管鳞状细胞癌中新的治疗靶点和空间异质性 发表期刊:nature commmunications 发表日期:2019年11月 影响因子:12.121 ...