T cell receptor (TCR) sequencing has emerged as a powerful new technology in analysis of the host鈥搕umour interaction. The advances in NextGen sequencing technologies, coupled with powerful novel bioinformatic tools, allow quantitative and reproducible characterisation of repertoires from tumour and ...
Quantitative T cell repertoire analysis by deep cDNA sequencing of T cell receptor α and β chains using next-generation sequencing (NGS). OncoImmunology 2014. Highly Clonal Regulatory T cell population in Follicular Lymphoma – Inverse Co...
The sequencing set-up is highly adaptable, employing configurations such as Illumina MiSeq PE300 and HiSeq PE150/250. A complete T-Cell Receptor (TCR) analysis is carried out, harnessing the principles of the 5'RACE technology. This enables the acquisition of full TCR sequences, facilitating ...
[8] Pai J A, Satpathy A T. High-throughput and single-cell T cell receptor sequencing technologies[J]. Nature methods, 2021, 18(8): 881-892. [9] Jiang, X., et al., Comprehensive TCR repertoire analysis of CD4(+) T-cell subsets in rheumatoid arthritis. J Autoimmun, 2020. 109: p...
标题:Multi-region sequencing unveils novel actionable targets and spatial heterogeneity in esophageal squamous cell carcinoma 多区域测序揭示了食管鳞状细胞癌中新的治疗靶点和空间异质性 发表期刊:nature commmunications 发表日期:2019年11月 影响因子:12.121 ...
TCR sequencing and analysis Successful amplification of the TCRβ region was verified by electrophoresis on a 2% agarose gel and followed by gel purification of the TCRβ bands using the Buffer QG and the QIAquick 96 PCR Purification kit (Qiagen), according to the manufacturer’s instructions. ...
High-throughput 3′ single-cell RNA-sequencing (scRNA-seq) allows cost-effective, detailed characterization of individual immune cells from tissues. Current techniques, however, are limited in their ability to elucidate essential immune cell features, including variable sequences of T cell antigen ...
bioinformaticsdata-analysisimmunologytcrigbioinformatics-analysisimmunoinformaticstcr-repertoireig-repertoire UpdatedJun 8, 2020 R fajrinard/Line-TCR Star24 Line Bot TCR pythontcrbot-tcr UpdatedNov 30, 2021 Python Code for "Attentive Variational Information Bottleneck for TCR-peptide Interaction Prediction", ...
and comparative analysis using next-generation sequencing is used to identify candidate TCRs recognizing an antigen encoded by one of the expressed TMGs. td: transduced.bSchematic of a combinatorial TCR library cloning strategy. An assembly reaction using multiple TCRα and β chains is performed, ...
[4] Thapa DR, Tonikian R, Sun C, Liu M, Dearth A, Petri M, Pepin F, Emerson RO, Ranger A. Longitudinal analysis of peripheral blood T cell receptor diversity in patients with systemic lupus erythematosus by next-generation sequencing. Arthritis Res Ther. 2015 May 23;17(1):132. doi:...