Our TCR-Seq service combines popularnext-generation sequencing (NGS)andbioinformaticstechnologies to create an unprecedented high-resolution image of the TCR repertoire, providing immense potential in uncovering the dynamic changes in clonal populations during antigen stimulation. Our TCR-Seq strategy entails...
Next-generation sequencing (NGS) is rapidly advancing immunology and immuno-oncology research, with the latest solution enabling researchers to study T-cell receptor (TCR) convergence accurately. Read the white paper to learn about TCR convergence and its emergence as a potential biomarker. Understand ...
TCRβ sequencing and phenotyping of single HIV-infected cells We developed a novel approach, using the unique VDJ rearranged sequence of TCRβ as a cellular tag, to track individual HIV-infected cells (Fig.1a). CD4+ T cells isolated from the blood of virally suppressed individuals were stimula...
The SMARTer Human scTCR Demultiplexer software* is only for use with the sequencing data generated with theSMARTer Human scTCR a/b Profiling Kit. CLC Genomics can also be used for demultiplexing. *Note: This is a different software package than theICELL8 scTCR Analyzerused with the human TCR...
The other aliquot was first stimulated with S-peptides pool, in the presence of IL-2 for 12 days, to promote the expansion of S-specific cells, and then was also subjected to TCRβ sequencing (post-stimulation repertoires). TCRβ repertoire analysis involved a filtering step to exclude ...
Genomic Sequencing of B2M in the TCR/B2M Knockout NFAT-Luciferase Reporter Jurkat cells Stimulation of NFAT Luciferase activity with anti-CD3 Agonist Antibody Stimulation of NFAT-Luciferase activity by Ionomycin/PMA Previous Next You may also be interested in ...
Flexible sequencing options:either full-length V(D)J information on the MiSeq™ system or CDR3 information on all Illumina platforms Figure 1. Sensitive and reproducible clonotype detection from a broad range of RNA amounts.TRAandTRBlibraries were generated from 1, 10, and 100 ng of human ...
Sequencing can be performed on an Illumina MiSeq sequencer using the 150-cycle with paired- end, 2 x 75 base pair reads. Customer Service/Ordering tel: 800.662.2566 (toll-free) fax: 800.424.1350 (toll-free) web: takarabio.com/service e-mail: ordersUS@takarabio.com Contact Us Technical ...
We performed high-throughput and paired γδ TCR sequencing of single intraepithelial γδ T cells of untreated CeD patients (n = 8; 1821 cells), CeD patients treated with a gluten-free diet (n = 5; 436 cells) and controls (n = 7; 1068 cells). We found that CeD ...
Florentin; for TCR sequencing, B. Paria and V. Hill; for retroviral vector production, S. Feldman, A. Cuenca, A. Mason, N. Gill, Q. Richburg and T. Novsak; for T cell product production, R. Somerville, Z. Franco, L. Parker, A. Nahvi, M. Langhan, T. Shelton, H. Xu, N....