Single-cell full-length TCR/BCR assays provide the ability to profile the highly diverse T cell receptor (TCR) and B cell receptor (BCR) repertoires of the adaptive immune response. The identification of T cell clonotype can be challenging due to insufficient sensitivity to detect low abundance ...
run-trust4 -f hg38_bcrtcr.fa --ref human_IMGT+C.fa -u path_to_10X_fastqs/*_R2_*.fastq.gz --barcode path_to_10X_fastqs/*_R1_*.fastq.gz --readFormat bc:0:15 --barcodeWhitelist cellranger_folder/cellranger-cs/VERSION/lib/python/cellranger/barcodes/737K-august-2016.txt [other ...
作者利用单细胞RNA以及BCR/TCR测序对急性KD患者PBMCs进行分析,通过静脉注射免疫球蛋白(IVIG)治疗前后不同细胞类型的差异表达分析,揭示单核细胞是炎症介质的主要来源,并且可能是治疗靶点。通过对比IVIG治疗后免疫细胞类型的动态,BCRs和TCRs出现克隆扩增,说明血浆细胞和效应记忆CD8+ T细胞也参与到KD中。本研究发现的先天和...
GEM-X Single Cell Immune Profiling v3 (5’ v3) allows you to maximize biological insights from clinical samples. Renal cell carcinoma dissociated tumor cells were loaded separately onto a Next GEM Chip K (10,000 cells) and a GEM-X 5' chip (20,000 cells), and then processed on a Chromi...
Multiomic readouts from the same cell 3' or 5' gene expression TCR/BCR Protein CRISPR For cost-effective studies Up to 160,000 cells (1-8 samples) per run, plus lower cost of <$600/sample Products Flex Gene Expression Fix, batch, and run on your schedule. ...
Mechanisms underpinning the dysfunctional immune response in severe acute respiratory syndrome coronavirus 2 infection are elusive. We analyzed single-cell transcriptomes and T and B cell receptors (BCR) of >895,000 peripheral blood mononuclear cells
TCR/BCR 抗原特异性 独特的样本制备能力 更多实验选项 基因表达 覆盖全转录 通过捕获mRNA转录本的3’或5’端分析整个转录组,对多个物种的细胞类型和状态进行无偏表征。 兼容的样本类型 细胞悬液、细胞核悬液、流式分选的细胞、固定的人类PBMC、新鲜组织、冷冻组织、DSP/甲醇固定的组织、类器官 ...
Evercode TCR and Evercode BCR are the only fixation-based scRNA-Seq Immune profiling assays available. TCR 10K-1M Cells 1-96 Samples Detect more TCR chains along with lowly expressed genes. Avoid ambient RNA contamination found in droplet-based single cell sequencing. ...
Comprehensive multi-omics single-cell data integration reveals greater heterogeneity in the human immune system. iScience 25, 105123 (2022). Article CAS PubMed PubMed Central Google Scholar He, J. et al. Research progress on application of single-cell TCR/BCR sequencing technology to the tumor ...
Waldenstrom’s Macroglobulinemia (WM) is an IgM-secreting bone marrow (BM) lymphoma that is preceded by an asymptomatic state (AWM). To dissect tumor-intrinsic and immune mechanisms of progression, we perform single-cell RNA-sequencing on 294,206 BM tumo