Single cell RNA-seq and ATAC-seq analysis of cardiac progenitor cell transition states and lineage settlementFormation and segregation of cell lineages forming the heart have been studied extensively but the underlying gene regulatory networks and epigenetic changes driving cell fate transitions during ...
none of the few read simulators aim to mimic real data. To fill this gap, we introduce scReadSim, a single-cell RNA-seq and ATAC-seq read simulator that allows
在关于利用Single-CellRNA-Seq来确定T细胞是否被激活选用的指标时,作者表示早期激活指标选用IFN-γ,IL-2备选(4小时);4-1BB(CD137)可作为晚期激活指标(16-48小时)。 最后,作者表示虽然本研究有效提高了TCRs筛选的效率,但依然需要花费较多的人力和时间,其中制备GMP级别的细胞需要3-5个月,而针对肿瘤突变抗原的RNA...
ezSingleCell平台的单细胞多组学(Single cell multiomics)模块通过整合单细胞RNA测序、ATAC-seq和蛋白质组学数据,提供全面的生物学分析解决方案(图7)。该模块支持多种数据格式的输入,并利用高级算法消除不同组学数据间的批次效应,确保分析结果的一致性和可靠性。用户可以在统一界面中进行数据预处理、降维和聚类分析,探...
ezSingleCell平台的单细胞多组学(Single cell multiomics)模块通过整合单细胞RNA测序、ATAC-seq和蛋白质组学数据,提供全面的生物学分析解决方案(图7)。该模块支持多种数据格式的输入,并利用高级算法消除不同组学数据间的批次效应,确保分析结果的一致性和可靠性。用户可以在统一界面中进行数据预处理、降维和聚类分析,探...
终于要开始分析scATAC-seq数据了,联合scRNA-seq就可以做multi-omics,可以深入挖掘TF的调控机制。 先从seurat和signac开始上手 cellRanger - Understanding Output 10x - ATAC Data Concepts 10x - Cell Ranger ATAC Algorithms Overview Integrating scRNA-seq and scATAC-seq data ...
Evaluation of Ranzoni et al.: Integrative Single-Cell RNA-Seq and ATAC-Seq Analysis of Human Developmental Hematopoiesis This article shows an example of the peer review process for "Integrative Single-Cell RNA-Seq and ATAC-Seq Analysis of Human Developmental Hematopoiesis" (Ranzoni et al., 2021...
or scRNA-seq+scATAC-seq from a single-cell multiomics experiment, where each cell is measured using both assays, and measurements are linked by a shared cell barcode. We demonstrated MIRA using SHARE-seq data and commercial 10X genomics multiome data, but MIRA's assumptions and models are ext...
On the other hand, healthy people can use single-cell sequencing to check regularly to achieve the purpose of early diagnosis Full size image Nevertheless, bulk RNA sequencing, which is the main technology of NGS, can only measure average gene expression, encompassing all individual cells and ...
CROP-seq 2017年7月,奥地利研究人员在Nature Methods上发表“Pooled CRISPR screening with single-cell transcriptome readout (doi:10.1038/ nmeth.4227)”文章,创造性地将CRISPR-Cas9和单细胞RNA测序两种极有前景的基因组学领域结合在一起,发明了这种被称作CROP-seq的单细胞测序方法 (CRISPR droplet sequencing, CRI...