Here, we profiled gene expression and chromatin accessibility in developing pig somites and myotomes at single-cell resolution. Results:We identified myogenic cells and other cell types and constructed a differentiation trajectory of pig skeletal muscle ontogeny. Along this trajectory, the dynamic ...
hematopoiesismulti-fluorescent mouse modelepigenetic memorylineage tracingRNA-seqsingle cellATAC-seqtransplantationStem cells determine homeostasis and repair of many... VWC Yu,Xin Wang,E Lee - 《Cell》 被引量: 40发表: 2016年 Single-cell epigenomics maps the continuous regulatory landscape of human he...
RNA-sequencing (RNA-seq) has become an increasingly cost-effective technique for molecular profiling and immune characterization of tumors. In the past decade, many computational tools have been developed to characterize tumor immunity from gene expressi
Here, we used integrative single-cell RNA-seq and ATAC-seq on adult insectivorous (Eptesicus fuscus; big brown bat) and frugivorous (Artibeus jamaicensis; Jamaican fruit bat) bats to identify cell populations, genes, and regulatory element differences that could potentially be associated with frugivo...
Simulated single cell RNA-seq and ATAC-seq data(Walkthrough): This simulated data were generated based on bulk RNA-seq and DNase-seq profiles from the same sample using MOSim package. Simulated single cell RNA-seq and ATAC-seq dataset 8(Walkthrough): This simulated data set consists of five...
We demonstrate its performance on multi-modality data of gene expression and chromatin accessibility and illustrate the integration abilities of Cobolt by jointly analyzing this multi-modality data with single-cell RNA-seq and ATAC-seq datasets.
(ATAC-seq [61], RNA-seq [62], mass spectrometry [63]; BCR and TCR sequencing [64]) but can be performed in parallel to single-cell assays; spatially resolved cells can be extracted from multiple platforms, sequencing or imaging-based (Spatial ATAC [38], Spatial CITESeq [65], Visium ...
Simultaneous measurements of transcriptomic and epigenomic profiles in the same individual cells provide an unprecedented opportunity to understand cell fates. However, effective approaches for the integrative analysis of such data are lacking. Here, we
To further elucidate the potential mechanism underlying the beneficial effect of FOT1 in MASH development, we first performed RNA sequencing (RNA-seq) analysis on both ND-fed mice and HID-fed mice. Lipid metabolism and fatty acid metabolism were among the top 5 enriched pathways, as shown by ...
2 Materials and methods 2.1 Public data collection The scRNA-seq data of five TNBC patients with primary tumors were collected from the Gene Expression Omnibus database. GSE169246 [9] (PD-L1 blockade-induced temporal single cell dynamics in TNBC), which utilized single-cell RNA- and ATAC-seque...