单细胞核转录组测序(singlecellnuclearRNASequencing)参考哪些检测标准啊? 单细胞核转录组测序(singlecellnuclearRNASequencing)检测周期需要多久呀? 单细胞核转录组测序(singlecellnuclearRNASequencing)对样品有什么要求呢? 单细胞核转录组测序(singlecellnuclearRNASequencing)可以测试具体哪些项呀? 单细胞核转录组测序(singl...
cell-associated genes (Fig.2a, b). Of note, the majority of immune cell transcripts within this sub-cluster were derived from the MCAO ipsi datasets (Supplementary Fig.8e, Supplementary notes). Importantly, previous research has shown that oligodendrocyte transcripts accumulate in the nuclear compa...
In plants, single-cell sequencing is still challenging. We have developed a simple method to isolate and sequence the whole genome of each of the four microspores from a plant tetrad21. In the present study, we make further efforts to improve the method for isolating the three nuclei from ...
lysis, reverse transcription (RT), complementary DNA (cDNA) amplification, library construction, sequencing, and data analysis [16] (Fig.1). Although capturing single cells quickly and accurately with high efficiency may seem trivial, it is one of the main challenges of single-cell sequencing...
Genetic studies of Alzheimer disease (AD) have prioritized variants in genes related to the amyloid cascade, lipid metabolism, and neuroimmune modulation. However, the cell-specific effect of variants in these genes is not fully understood. Here, we perform single-nucleus RNA-sequencing (snRNA-seq...
Germ-cell nuclear antigen hPGC: Human primordial germ cell KS: Klinefelter syndrome MMLV: Moloney murine leukemia virus NOA: Non-obstructive azoospermia OA: Obstructive azoospermia PGC: Primordial germ cell SCOS: Sertoli cell only syndrome scRNA-seq: ...
To fully understand the context-specific function of involved nuclear processes, a vast number of single-cell sequencing technologies were developed over the last decade. This instrumental work demonstrated the heterogeneity between cell types and individual cells, bringing about new understanding of ...
Nuclear RNA-seq of single neurons reveals molecular signatures of activation Single-cell sequencing methods have emerged as powerful tools for identification of heterogeneous cell types within defined brain regions. Application of single-cell techniques to study the transcriptome of activated neurons can ...
Single cardiomyocyte nuclear transcriptomes reveal a lincRNA-regulated de-differentiation and cell cycle stress-response in vivo. Nat. Commun. 8, 225 (2017). Article PubMed PubMed Central CAS Google Scholar Gao, R. et al. Nanogrid single-nucleus RNA sequencing reveals phenotypic diversity in ...
which was because that the macula samples collected for this study did not contain the fovea, where cone cells have a much more increased proportion. Since snRNA-seq is less biased in sampling in comparison to single-cell sequencing, a better estimation of cell proportion can be obtained. By ...