在本周一的文章(复现原文(一):Single-cell RNA sequencing of human kidney(step by step))中,我们完成了scRNA-seq数据的质控(Hemberg-lab单细胞转录组数据分析(三)- 原始数据质控)、批次校正、找Marker基因(单细胞分群后,怎么找到Marker基因定义每一类群?)、UMAP可视化和tSNE可视化(Hemberg-lab单细胞转录组数据分...
Single-cell sequencing technologies can be used to detect the genome, transcriptome and other multi-omics of single cells. They can show the differences and evolutionary relationships of various cells. This review introduces the latest advances in single-cell sequencing technologies and their applications...
In this study, we used single-cell RNA-sequencing (scRNAseq) to define the molecular logic by which SGN subtypes diversify in the mouse. Our detailed analysis of transcriptional dynamics of SGNs from several embryonic and perinatal stages indicates that neuronal subtypes successively emerge during HC...
Droplet microfluidic methods have massively increased the throughput of single-cell sequencing campaigns. The benefit of scale-up is, however, accompanied by increased background noise when processing challenging samples and the overall RNA capture efficiency is lower. These drawbacks stem from the lack...
cell analysis of PDAC, Elyada et al. confirmed the presence of myofibroblastic CAFs (myCAFs) and inflammatory CAFs (iCAFs) using single-cell RNA sequencing and discovered a new CAF population, i.e., “antigen-presenting CAFs” (apCAFs) (Fig.2). By analysing the genetic profiles of these...
In this study, we performed single-cell RNA sequencing (scRNA-seq) to compare the gene expression pattern and associated pathways of each individual cell between anti-PD-L1 treated CRC samples with or without F. nucleatum infection. We then tracked the developmental trajectory of tumor cells to ...
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-specific markers and cell types. scRNA-seq plays...
First, single-cell sequencing is limited in few patients, which prevents us to access comprehensive overview and broadly inspect myeloma pathogenesis and drug resistance. Further investigation in large-scale MM samples is necessary. Second, our present study discovered some interesting changes of both ...
The cellular heterogeneity, metabolic features, interactions across ten tissue types of lactating dairy cattle were studied at single-cell resolution in the current study. Methods Unbiased single-cell RNA-sequencing and analysis were performed on the rumen, reticulum, omasum, abomasum, ileum, rectum, ...
To determine the sensitivity of our single-cell sequencing, we calculated the detection rate of each ERCC species (Additional file9: Figure S4E). With the exception of ERCC-00116, which is inefficiently sampled by polyA selection [33], at more than eight copies, ERCCs were detected in 99–...