在本周一的文章(复现原文(一):Single-cell RNA sequencing of human kidney(step by step))中,我们完成了scRNA-seq数据的质控(Hemberg-lab单细胞转录组数据分析(三)- 原始数据质控)、批次校正、找Marker基因(单细胞分群后,怎么找到Marker基因定义每一类群?)、UMAP可视化和tSNE可视化(Hemberg-lab单细胞转录组数据分...
Single-cell sequencing technologies can be used to detect the genome, transcriptome and other multi-omics of single cells. They can show the differences and evolutionary relationships of various cells. This review introduces the latest advances in single-cell sequencing technologies and their applications...
在本周一的文章(复现原文(一):Single-cell RNA sequencing of human kidney(step by step))中,我们完成了scRNA-seq数据的质控(Hemberg-lab单细胞转录组数据分析(三)- 原始数据质控)、批次校正、找Marker基因(单细胞分群后,怎么找到Marker基因定义每一类群?)、UMAP可视化和tSNE可视化(Hemberg-lab单细胞转录组数据分...
single-cell RNA sequencing has unique advantages and can help us gain a better understanding of different cell subsets. In a recent cross-species single-cell analysis of PDAC, Elyada et al. confirmed the
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-specific markers and cell types. scRNA-seq plays...
Paired single-cell TCRαβ/ γδ sequencing Single-cell TCRαβ sequencing library was generated using the Chromium Single Cell 5′ Library Construction kit (PN1000020, 10X Genomics) and Chromium Single Cell V(D)J Enrichment Kit for mouse T cell (PN-1000071, 10X Genomics). In all, 1/22.5...
In this study, we used single-cell RNA-sequencing (scRNAseq) to define the molecular logic by which SGN subtypes diversify in the mouse. Our detailed analysis of transcriptional dynamics of SGNs from several embryonic and perinatal stages indicates that neuronal subtypes successively emerge during HC...
cell subsets contributes to the immunosuppressive TME, leading to cancer progression and the failure of ICB. The application of single-cell RNA sequencing (scRNA-seq) provides insights into TME heterogeneity. Th1 cell, CD4+T helper 1 cell; NK cell, natural killer cell; TAM, tumor-associated ...
(environment). Cell-specific scaling factors, such as capture and RT efficiency, dropout/amplification bias, dilution factor, and sequencing amount, must be considered in the normalization step.bSingle-cell latent variable model (scLVM) can effectively remove the variation explained by the cell-...
Colorectal cancer Single-cell RNA sequencing PD-L1 blockade Fusobacterium nucleatum Immunotherapy 1. Introduction Though standard conventional treatments have made substantial progress, colorecatal cancer (CRC) ranked third of newly diagnosed cases and second of cancer-related deaths [1]. Owing to the bu...