开发出一种SCI-seq (single-cell combinatorial indexed sequencing,单细胞组合标记测序技术),多次对细胞进行条形码编码标记后对它们进行测序,可以同时构建上千个单细胞文库,检测体细胞拷贝数的变异。
multi-layered data sets that challenge the extraction of meaningful biological insights. A recognized impediment in single-cell sequencing is the “omission”—the omission of certain cell types due to technological constraints on the sequencing platform and interruption of the trajectory of cell differen...
BCMA-targeted CAR-T cell therapy for multiple myeloma. Worldwide patent WO2022116086A1 (2022). Germain, P.-L., Lun, A., Meixide, C. G., Macnair, W. & Robinson, M. D. Doublet identification in single-cell sequencing data using scDblFinder. F1000Res. 10, 979 (2022). Aran, D...
For more informations please refer to the manuscript:Aran, Looney, Liu et al. Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage. Nature Immunology (2019) Install devtools::install_github('dviraran/SingleR')# this might take long, though mostly beca...
indicating cell-type-specific expression, than canonical introns (P < 2.2 × 10−308,t = −55.874, d.f. = 40,909; Extended Data Fig.2a). Furthermore, we sequenced complementary DNA (cDNA) from four donors with PacBio multiplexed arrays sequencing (MAS-seq); 85.9%...
The resulting developmental atlas provides a single-cell RNA sequencing (scRNA-seq) map of an inflorescence. We validated our results by mRNA in situ hybridization and by fluorescence-activated cell sorting (FACS) RNA-seq, and we show how these data may facilitate genetic studies by predicting ...
Single-cell sequencing (scRNA-seq) has provided new insights into the analysis of TAMs. Nearly all macrophage subtypes which exhibit distinct marker genes as well as vary in morphology and function within the TME co-express M1 and M2 characteristics [12]. The discovery of TAMs further reveals ...
Single Cell Portal overview Please note the portal and wiki are in Beta! Your feedback is so valuable and thank you! Welcome! The Single Cell Portal was developed to facilitate sharing scientific results, and disseminating data generated from single cell technologies. We strive to make open scien...
As a revolutionary technology, single-cell RNA sequencing (scRNA-seq) has become the preferred method for determining the composition of complex tissues at the transcriptional level [12]. In contrast to traditional bulk RNA sequencing, scRNA-seq can reveal new cell types and rare subpopulations and...
Single‑cell RNA sequencing analysis The scRNA data was processed utilizing the R (v4.0.5) package Seurat (v4.0.2) [16]. The ensuing quality criteria were imposed: (1) genes discerned in fewer than 3 cells were omitted; (2) cells revealing fewer than 50 genes in total were disregarded...