Another dimension of advancement has been the emergence of long-read sequencing platforms that can sequence longer individual DNA molecules. These so-called “third generation” platforms, such as PacBio and Oxford Nanopore long read technologies, have evolved to a cost per acquired base that now r...
on delivering best-in-class accuracy, resulting in over 90 issued patents and numerous pending applications in the US and abroad. The Onso system utilizes SBB chemistry to free researchers from the limitations of existing short-read sequencing platforms, providing ≥90% of bases at Q40+ accuracy....
Clinical genomics laboratories will evaluate these platforms from a unique perspective, focusing on how technological advancements can improve patient care. CONTENT. This review describes short-read sequencing platforms provided by Illumina, Element Biosciences, MGI, PacBio, Singular Genomics, Thermo Fisher...
Sequencing-by-synthesis on Illumina’s platforms typically involves the following steps. First, multiple copies of the genome being sequenced are broken into short fragments in a random fashion, followed by ligation of sequencing adapters to the fragments. In the next phase, the DNA sample is intr...
Proprietary sequencing by binding (SBB) chemistry uses native nucleotides, scarless incorporation, and optimized conditions for binding and extension. These innovations result in data with decreased errors relative to competing platforms and, as a result, allow detection of rare variants below the limit...
The growing demand for sequencing has led to the recent emergence of several new sequencing platforms with techniques that may provide alternatives or improvements to existing workflows or allow the adoption of sequencing workflows in new settings. Clinical genomics laboratories will evaluate these ...
Keane T, Ning Z: Assessing Assemblability of Reads from New Sequencing Platforms. 15th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB) Poster 2007. [http://www.iscb.org/uploaded/css/B16Keane.pdf] Google Scholar Hernandez D, Francois P, Farinelli L, Osteras ...
launched lab services for whole genome sequencing-based services for genomic clinical assays for infectious disease diagnosis. In addition to this, GENEWIZ also provides custom short-read amplicon sequence analysis using Illumina, Inc. platforms for antibody profiling, genome editing, mutation analysis, ...
12, 13, 14, 15, 16 Pathogenic repeat expansions are usually significantly longer than the reads generated by short-read sequencing platforms such as Illumina and can be longer than the library insertion fragments. Therefore, the short reads cannot span many pathogenic repeat-expansion alleles, such...
We deep-sequenced the transcriptome of Douglas-fir needles from seedlings that were grown under nonstress control conditions or a combination of heat and drought stress conditions using long-read (LR) and short-read (SR) sequencing platforms. We used 2 computational approaches, namely de novo and...