2019年,根据2012的29例SCLC手术组织和2015年52例手术切除组织基因组测序数据,结合54株细胞系RNA测序数据,通过层次聚类分析,根据关键转录因子ASCL1、NEUROD1、和POU2F3的最大表达量可将SCLC分为A/N/P/N亚型[1]。2021年,使用单细胞RNA...
反应性星形胶质细胞向SCLC脑转移瘤迁移、浸润并促进其生长 反应性星形胶质细胞浸润 SCLC 脑转移灶:从SCLC脑异体移植物中分离的星形胶质细胞群,在scRNA-seq分析和胶质纤维酸性蛋白(GFAP)免疫染色中显示出反应性星形胶质细胞的特征,具有特征性肥大形态。与对照组相比,大量RNA-seq证实肿瘤相关星形胶质细胞发育程序富集。在...
📊 YAP1在SCLC中的表达定义了一个独特的亚型,具有T细胞炎症表型🔬 主要研究方法:RNA-seq和免疫组化,使用了discovery set和validation set。📚 主要收获: 🌱 小细胞肺癌(SCLC)根据转录因子的表达分为四种亚型:SCLC-A/N/Y/P,分别表达转录因子ASCL1、NeuroD1、YAP1及POU2F3。然而,这些亚型的临床和生物学意...
入组ES-SCLC 患者接受 4 个周期D+EP序贯度伐利尤单抗治疗,至多进行6个周期EP治疗。RNA-seq 数据来自筛选时收集的FFPE 肿瘤样本。数据截止日期为2021 年 3 月 22 日。 结果: D+EP组和EP组分别有 21.3% (57/268) 患者和17.5% ...
mrna资料透过rna测序分析正常组织和癌组织获得(参考实施例2、图2)。正常组织资料的额外来源是从3000个正常组织样本可公开获得的rna表达资料的资料库(lonsdale,2013)。获得自蛋白的肽在癌组织中显示高表达编码mrna,但是在重要正常组织中非常低或不存在,这些肽作为优选肽纳入本发明。 186、本发明提出了有利于治疗癌症/...
RNAseq data were generated from FFPE tumor samples collected at screening. Data cutoff: Mar 22, 2021. Results 57/268 (21.3%) patients in the D+EP arm, 60/268 (22.4%) patients in the D+T+EP arm, and 47/269 (17.5%) patients in the EP arm had RNAseq data (biomarker-evaluable ...
最后,根据肿瘤内异质性水平的普遍性,确定一种称为ITHtyper的基因特征,该特征能够根据大量RNA-seq图谱对患者进行风险分层。ITHtyper的预后价值在独立的多中心患者队列中得到了严格的验证。本研究介绍了一种初步的以肿瘤为中心的区域靶向空间转录组资源,揭示了 SCLC 中以前未探索的肿瘤内空间异质性。这些发现有望改善...
Human SCLC RNA-seq data ethical approval All SCLC and normal lung patient datasets and human SCLC cell line data used in this study have been previously published and, as such, are appropriately referenced, have previously obtained the appropriate ethics approvals and are available online under htt...
RNA was extracted with the Omega Mag-Bind FFPE RNA extraction kit (Omega Bio-Tek, Catalog M2551) according to the manufacturer’s protocol. RNA sequencing (RNA-seq) libraries were prepared by Omega Bioservices. RNA library preparation and sequencing: The RNA-seq libraries were prepared using Ill...
The RNA-seq results indicated the robust effect of combination Teri&Lur treatment on critical cancer pathways in an SBC3 spheroid model. Applying Teri and Lur at a low concentration range for 24 h increased cellular cytotoxicity, cell-cycle arrest, apoptosis, and DNA damage and suppressed cell-...