近日，来自德国Max Planck Institute of Biochemistry的Brenda A. Schulman研究组在Cell上发表题为Systemwide disassembly and assembly of SCF ubiquitin ligase complexes的文章，就上述问题进行了深入研究。作者通过冷冻电镜（cryo-EM）获得多种状态下结合有CAND1的SCF复合物结构，并进一步将结构、生物化学指标以及细胞生物...
An SCF-like ubiquitin ligase complex that controls presynaptic differentiation. Nature, 430(6997), pp.345-350. Available at: http://dx.doi.org/10.1038/nature02647.Liao EH, Hung W, Abrams B, Zhen M (2004) An SCF-like ubiquitin ligase complex that controls presynaptic differentiation. Nature ...
但是,这样单独一个因子是如何协调多种不同多蛋白复合物装配的,也就是SCF复合物是如何通过CAND1依赖的方式进行装配的,仍旧不清楚。 近日,来自德国Max Planck Institute of Biochemistry的Brenda A. Schulman研究组在Cell上发表题为Systemwide disassembly and ass...
Crystal structure of mammalian cryptochrome in complex with a small molecule competitor of its ubiquitin ligase. Cell Res. 23, 1417–1419 (2013). CAS PubMed PubMed Central Google Scholar Ye, J. et al. Disruption of hepatitis C virus RNA replication through inhibition of host protein ...
英文名称:Recombinant Talaromyces stipitatus E3 ubiquitin ligase complex SCF subunit sconC sconCRecombinant Talaromyces stipitatus E3 ubiquitin ligase complex SCF subunit sconC sconC is available 2 ,中文名称:重组Stiptatus Talaromyces E3泛素连
This result is in contrast to all other known SCF components, whose presence in the isolated complex is required for ubiquitin ligase activity in vitro. We cannot, however, exclude the possibility that insect Sgt1p plays a role in SCF activity in the recombinant system but is not a part of...
theyeastkinetochorecomplex,CBF3,viaactivational.,1996;ConnellyandHieter,1996;Kamuraetal.,1999). ofCtf13p.Sgt1palsoassociateswithSCF(Skp1p/BiochemicalstudiesrevealedthatSkp1pisacompo- Cdc53p/Fboxprotein)ubiquitinligase.sgt1–5(G1al- nentofSCF(Skp1p,Cdc53p,Fboxprotein)ubiquitin lele)mutantsaredefecti...
We previously reported that AGP1 induction requires Grr1, the F-box protein part of the SCF Grr1 ubiquitin–ligase complex. We show here that ubiquitin, other components of SCF Grr1 and the ubiquitin-conjugating enzyme Cdc34 are essential for AGP1 and PTR2 induction. This suggests that ...
The SCF ubiquitin E3 ligase regulates ubiquitin-dependent proteolysis of many regulatory proteins such as p27Kip1, IκB, and β-catenin. We report the isolation of a CUL1 binding protein, p120CAND1. We found the majority of CUL1 is in a complex with CAND1 and ROC1 independent of SKP1 ...
Bosco G, Du W, Orr-Weaver TL: DNA replication control through interaction of E2F-RB and the origin recognition complex. Nat Cell Biol. 2001, 3: 289-295. 10.1038/35060086. Article CAS PubMed Google Scholar Wojcik EJ, Glover DM, Hays TS: The SCF ubiquitin ligase protein slimb regulates ...