Currently, two major families of E3 ligases, the HECT and the RING families, have been described. Cullins are a family of evolutionarily-conserved proteins that assemble the largest number of distinct RING-type
Pellino proteins were initially characterized as a family of E3 ubiquitin ligases that can catalyse the ubiquitylation of interleukin-1 receptor-associated kinase 1 (IRAK1) and regulate innate immune signalling pathways. More recently, physiological and molecular roles for members of the Pellino family...
P. Structure of a c-Cbl–UbcH7 complex: RING domain function in ubiquitin–protein ligases. Cell 102, 533–539 (2000). CAS PubMed Google Scholar Dominguez, C. et al. Structural model of the UbcH5B/CNOT4 complex revealed by combining NMR, mutagenesis, and docking approaches. Structure ...
siRNA-mediated knockdown of the Cullin-RING E3 ubiquitin ligases RBX1 or CUL1 increased HES1 protein levels, prolonged its half-life, and dampened its oscillation. FBXL14, an F-box protein for SCF ubiquitin ligase, associates with HES1. FBXL14 silencing stabilized HES1, whereas FBXL14 ...
F-box proteins (FBPs) are components of the largest family of SCF-type (SKP1, Cullin1 and F-box protein) E3 ubiquitin ligases called SCF1 (SKP1, Cullin1 and F-box protein) or CRLs (Cullin-RING ligases) [1]. FBPs interact through their F-box domain with SKP1, which binds to a...
Control of cell growth by the SCF and APC/C ubiquitin ligases. Curr. Opin. Cell Biol. 21, 816–824 (2009). Article CAS PubMed PubMed Central Google Scholar Petroski, M. D. & Deshaies, R. J. Function and regulation of cullin-RING ubiquitin ligases. Nat. Rev. Mol. Cell Biol. 6,...
There are more than 600 types of E3 ubiquitin ligases that have been found. Classically, these E3 can be classified into three main types, RING (really interesting new gene) type, HECT (homologous to E6AP C-terminus) type, and RBR (RING-between-RING) type, respectively [16,17] (Fig.2...
insights into the structure and assembly of ECS-type Cullin-RING E3 Ubiquitin Ligases, Proteins of the ankyrin-repeat and SOCS-box (ASB) family act as the substrate-recognition subunits of ECS-type (ElonginBC-Cullin-SOCS-box) Cullin RING... JC Thomas,D Matakvinkovic,I Van Molle,... 被引...
SCF ubiquitin ligases share the core subunits cullin 1, SKP1, and HRT1/RBX1/ROC1, which associate with different F-box proteins. F-box proteins bind substrates following their phosphorylation upon stimulation of various signaling pathways. Ubiquitin-mediated destruction of the fission yeast cyclin-de...
Cullin-RING ubiquitin E3 ligases (CRLs) are regulated by modification of an ubiquitin-like protein, Nedd8 (also known as Rub1) on the cullin subunit. Neddylation is shown to facilitate E3 complex assembly; while un-neddylated cullins are bound by CAND1 that prevents recruitment of the substra...