TRAIL-R2 (DR5) is a clinically-relevant therapeutic target and a key target for immune effector cells. Herein, we identify a novel interaction between TRAIL-R2 and the Skp1-Cullin-1-F-box (SCF) Cullin-Ring E3 U
The full enzymatic activity of the cullin-RING ubiquitin ligases (CRLs) requires a ubiquitin-like protein (that is, Nedd8) modification. By deamidating Gln40 of Nedd8 to glutamate (Q40E), the bacterial cycle-inhibiting factor (Cif) family is able to inhi
Mitotic progression is also regulated by another ubiquitin ligase complex, the APC/cyclosome. We have tested mutations in SKP1, SGT1, and various APC subunit genes for synthetic interactions, but there is currently no evidence for genetic interactions. In addition, Pds1p, a target of the APC,...
The SCF ubiquitin E3 ligase regulates ubiquitin-dependent proteolysis of many regulatory proteins such as p27Kip1, IκB, and β-catenin. We report the isolation of a CUL1 binding protein, p120CAND1. We found the majority of CUL1 is in a complex with CAND1 and ROC1 independent of SKP1 ...
oftheSCFUbiquitinLigaseComplex mutationsinanyoneofthemabolishtheCDEIII-binding activityofCBF3. SKP1wasidentifiedasahighcopysuppressorofa ctf13mutant,ctf13–30(ConnellyandHieter,1996),and asasuppressorofcdc4–1(Baietal.,1996)andhas KatsumiKitagawa,*DorotaSkowyra, † StephenJ.Elledge, †‡ J.Wade...
The F-box protein Fbxw7 is a component of SCFFbxw7, a Skp1-Cul1-F-box E3 ubiquitin ligase complex that tags specific proteins for proteasome ... Martin,Hagedorn,Maylis,... - 《Cell Division》 被引量: 118发表: 2007年 A new subfamily of structurally related human F-box proteins. F-...
(PAMPs)and virus. Based on the GeneChip results,a group of E3 ubiqnitin ligase complex whose function in innate anti.viral immune responses remain unsown attract our attention.As F-BOX protein is the key substrate recongnition component of this complex,we analyse F-BOX proteins’expression ...
The VHL gene product (pVHL) forms a multimeric complex with the elongin B and C, Cul2 and Rbx1 proteins (VCBCR complex), which is homologous to the SCF family of ubiquitin ligase complexes. The VCBCR complex binds HIF-1α and HIF-2α, transcription facto
We show here that Emi1 destruction in early mitosis requires the SCFβTrCP ubiquitin ligase complex. Binding of βTrCP to Emi1 in late prophase requires a DSGxxS consensus phosphorylation motif, similar to the DSGxxS binding motifs seen in β-catenin and IκB, other known βTrCP targets ...