Cover the plate with an adhesive plastic and incubate for 2 hours at room temperature. Wash the plate 4 times with solution. Add 100µl of conjugated secondary antibody diluted to the optimal concentration (according to manufacturer datasheet). Dilute with blocking buffer immediately before use. ...
PathScan® Phospho-Stat3 (Tyr705) Sandwich ELISA Antibody Pair 规格 1 Kit 货号 GOYK96481 一抗和二坑的区别: *抗体就是平常所说的抗体,即能和抗原特异性结合。 *抗体是能和抗体结合的,即抗体的抗体。主要用于检测抗体的存在。 一抗是针对抗原的抗体,二抗是针对一抗的抗体。即抗体也可以充当抗原刺激机体...
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A purified rabbit IgG fraction against protoscolex excretory鈥搒ecretory products was used as primary antibody, and the same fraction conjugated with biotin as secondary antibody. In order to validate the sandwich ELISA, intra- and inter-assay precision, linearity, and recovery percentages were ...
An indirect ELISA using purified sIRAP (4 µg/ml) was conducted to compare the pre-subcloned (pre-SC) antibodies with the newly subcloned antibodies which were either untagged or biotinylated (detected using anti-Ms-HRP secondary antibody or streptavidin-HRP). All antibodies were used at 1 ...
CST's PathScan® Phospho-HER2/ErbB2 (Tyr1221/1222) Sandwich ELISA Antibody Pair is offered as an economical alternative to our PathScan® Phospho-HER2/ErbB2 (Tyr1221/1222) Sandwich ELISA Kit #7148. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody...
A sensitive and specific monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (ELISA) was established and validated for the detection of staphylococcal enterotoxin A (SEA). After routine fusion and selection, 10 monoclonal antibodies showed high affinity for SEA. An optimal pair for sand...
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The aim of the present study was to develop a rapid and accurate method for the detection of Riemerella anatipestifer based on a double antibody sandwich ELISA.Purified polyclonal antibody against serotype 1 R.anatipestifer was used as a capture antibody,while purified monoclonal antibody 5G7 ...
ELISA双抗夹心法1. Objective:To establish antibody_sandwich ELISA method for detection of Mycoplasma genitalium (Mg) antigen and evaluate its value for clinical application. 方法:采用不同株Mg单抗用ELISA双抗夹心法检测Mg抗原 ,摸索该方法检测Mg抗原的最佳实验条件。