By using the invention, a double-antibody sandwich ELISA method can be conveniently utilized to detect the Eperythrozoon suis. 在本发明的基础上,可方便地应用双抗夹心ELISA法检测猪附红细胞体。 ip.com 2. Dog feces were tested by double antibody sandwich ELISA method to confirm the coproantigen ...
The invention relates to a double-antibody sandwich ELISA (enzyme linked immuno-sorbent assay) test method for ovalbumin and belongs to the field of biological inspection and quarantine. The method comprises the following steps: preparing monoclonal antibodies capable of resisting ovalbumin; and ...
Development of Double-Antibody Sandwich Indirect ELISA to Detect Deer Blood鹿血双抗体夹心间接ELISA检测方法的建立鹿血ELISA多克隆抗体通过制备兔抗鹿血和小鼠抗鹿血多克隆抗体,建立了以兔抗为包被抗体,鼠抗为检测抗体的鹿血双抗体夹心间接ELISA检测方法。采用方阵滴定法确定了抗原、抗体的最佳反应浓度,并对ELISA各反应...
双抗体夹心酶联免疫吸附法检测杏仁过敏原苦杏仁球蛋白张洁琼,高淑霞,生威,张燕,王硕宰( 天津科技大学食品工程与生物技术学院,教育部食品营养与安全重点实验室,天津300457)摘要;提取中国甜杏仁过敏原蛋白苦杏仁球蛋白,分别制备兔和鼠多克隆抗体,建立双抗体夹心酶联免疫吸附( ELISA) 法。结果表明:对甜杏仁中苦杏仁球蛋...
Development of a Double-Antibody Sandwich Elisa for Rapid Detection of Bacillus Cereus in Food. Sci. Rep. 6, 16092 (2016).L. Zhu, J. He, X. Cao, K. Huang, Y. Luo, and W. Xu, "Develop- ment of a double-antibody sandwich ELISA for rapid detection of Bacillus Cereus in food," ...
a test for antigen; an application of the ELISA method in which material being tested for antigen is added to wells coated with known antibody; the presence of antigen fixed to the antibody coat can be determined either directly, by adding antibody linked to the enzyme of the indicator system...
Double antibody sandwich ELISA( DAS-ELISA) method was developed by using purified rabbit anti Po IFN-α Ig G as capture antibody,and purified mouse anti Po IFN-α Ig G as detection antibody. The result indicated that the best package concentration of rabbit anti porcine Po IFN-α Ig G was...
采用双抗体夹心酶联免疫吸附法(ELISA)测定尿中Tamm-Horsfall蛋白的含量。 pp.lunwenchina.net.cn 3. DogfecesweretestedbydoubleantibodysandwichELISAmethodto confirmthecoproantigenpositiverate. 收集牧民的家犬粪便,用双抗体夹心法检测粪抗原阳性率。 www.jsczz.cn:8080 ...
The results showed that the specificity and sensitivity of DAS-ELISA were 98.1% and 80.0%,respectively,and the agreement ratio of DAS-ELISA to RT-PCR was 95.8%. 展开 关键词: Cysticercus cellulosae scolex protein double antibody sandwich ELISA monoclonal antibody ...
The aim of the present study was to develop a rapid and accurate method for the detection of Riemerella anatipestifer based on a double antibody sandwich ELISA.Purified polyclonal antibody against serotype 1 R.anatipestifer was used as a capture antibody,while purified monoclonal antibody 5G7 ...