Sandwich ELISA analysis of human IgE was performed by coating wells of a 96-well plate with100 µL per well of a goat anti-human IgG (H+L) antibody (Product # 31119) diluted to a concentration of 1 µg/mL in carbonate/bicarbonate buffer (Product # 28382), and incubating overnight...
Human IgE Secondary Antibody (MII0201) in ELISA Direct ELISA analysis of human IgE was performed by coating wells of a 96-well plate with 100 µL per well of human IgE, IgM, IgA, or IgG, diluted in carbonate/bicarbonate buffer (Product # 28382) at concentrations ranging from 5-5000 ng...
Mouse IgG (H+L) Secondary Antibody (SA5-10212) in ELISA ELISA of mouse immunoglobulins shows RMG07 reacts to mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, and rabbit IgG. The plate was coated with 50 ng/well of different immunogl...
IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. 数据 Human IgG (Light chain) Secondary Antibody (SA5-10224) in ELISA
A secondary antibody aids in the detection, sorting or purification of target antigens by binding to the primary antibody, which directly binds to the target antigen. Since, the vast majority of primary antibodies are produced in just a f...
Applications:ELISA, WB, Dot blot Non visible text Multi-rAb CoraLite® Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) Conjugate:CoraLite® Plus 750 Applications:WB (Fluorescent), IF, FC Non visible text 1 Publications
Secondary Antibody Range NEW Multi-rAb Recombinant Secondary Antibodies are mixtures of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on the same IgG. Each recombinant clone in the final multiclonal secondary antibody mixture is carefully selected after rig...
1. Target specificity– The secondary antibody must be raised against the host species of the primary antibody. For example, if the primary antibody was generated in mouse, the secondary must be anti-mouse and raised in a host species other than mouse (e.g., goat anti-mouse secondary antibo...
Proteintech offers a comprehensive portfolio of secondary antibodies for detecting unconjugated primary antibodies in a wide range of applications including western blot, ELISA, immunofluorescence, immunohistochemistry, and flow cytometry. Explore All Secondary Antibodies Choosing a Secondary Antibody The choice...
For example, if the primary antibody is made in mouse, an “Anti-Mouse” secondary antibody is required. Select the desired target species from the Target dropdown in the Product Filter. Note: Both anti-Syrian and anti-Armenian hamster secondary antibodies are listed under “Anti-Hamster”. ...