在本发明的基础上,可方便地应用双抗夹心ELISA法检测猪附红细胞体。 ip.com 2. Dog feces were tested by double antibody sandwich ELISA method to confirm the coproantigen positive rate. 收集牧民的家犬粪便,用双抗体夹心法检测粪抗原阳性率。 www.jsczz.cn:8080 3. Application of the double antibody sa...
The invention relates to a double-antibody sandwich ELISA (enzyme linked immuno-sorbent assay) test method for ovalbumin and belongs to the field of biological inspection and quarantine. The method comprises the following steps: preparing monoclonal antibodies capable of resisting ovalbumin; and ...
Objective To establish a double-antigen sandwich ELISA(S-ELISA) for detection of total antibodies against hepatitis C virus(HCV).Methods Recombinant HCV proteins fusion-expressed with His-tag and GST-tag were separately used as coating and HRP-labeling antigen of the S-ELISA.Serum samples were te...
Development of Double-Antibody Sandwich Indirect ELISA to Detect Deer Blood鹿血双抗体夹心间接ELISA检测方法的建立鹿血ELISA多克隆抗体通过制备兔抗鹿血和小鼠抗鹿血多克隆抗体,建立了以兔抗为包被抗体,鼠抗为检测抗体的鹿血双抗体夹心间接ELISA检测方法。采用方阵滴定法确定了抗原、抗体的最佳反应浓度,并对ELISA各反应...
by Ni-NTA metal-affinity chromatography, which was identified by Western blot and ELISA, then we set up the double antigen sandwich ELISA for detection of anti-HDV in human serum, and the performance of the sandwich ELISA was evaluated in terms of specificity and sensitivity. Results were: 1...
African swine fever virus;double-antigen sandwich ELISA;diagnosis;p30 protein;prokaryotic expression system 2. Materials and Methods 2.1. Serum Samples Positive standard sera for ASFV, pseudorabies virus (PRV), classical swine fever virus (CSFV), and porcine circovirus type 2 (PCV2) were purchased ...
双抗体夹心酶联免疫吸附法检测杏仁过敏原苦杏仁球蛋白张洁琼,高淑霞,生威,张燕,王硕宰( 天津科技大学食品工程与生物技术学院,教育部食品营养与安全重点实验室,天津300457)摘要;提取中国甜杏仁过敏原蛋白苦杏仁球蛋白,分别制备兔和鼠多克隆抗体,建立双抗体夹心酶联免疫吸附( ELISA) 法。结果表明:对甜杏仁中苦杏仁球蛋...
Double antibody sandwich ELISA( DAS-ELISA) method was developed by using purified rabbit anti Po IFN-α Ig G as capture antibody,and purified mouse anti Po IFN-α Ig G as detection antibody. The result indicated that the best package concentration of rabbit anti porcine Po IFN-α Ig G was...
a test for antigen; an application of the ELISA method in which material being tested for antigen is added to wells coated with known antibody; the presence of antigen fixed to the antibody coat can be determined either directly, by adding antibody linked to the enzyme of the indicator system...
ELISA were as follows:polyclonal antibody with 184 ng per well was added to coat overnight,the plate was blocked by 100 mL/L neonatal calf serum for 2 hours at 37 ℃,the incubation times for detection antigen,monoclonal antibody and second labeled antibody were 1 hour at 37 ℃ for each,...