A direct sandwich anti-bovine IgG ELISA has been developed for the sensitive and specific detection of cow milk in adulterated human breast milk. This assay uses polyclonal anti-bovine IgG antibody as a capture antibody and monoclonal anti-bovine IgG-alkaline phosphatase antibody as a detection ...
A sandwich ELISA with specificity for a major antigen (Ag 7) of Aspergillus fumigatus has been compared with indirect ELISAs with use of crude antigenic (culture filtrate) extracts and found to have a sensitivity of 100% and specificity of 92.3% for antibody detection in sera of patients with...
Enzyme linked immunosorbent assays (ELISA) were developed to detect antigens from Phoneutria nigriventer spider venom. Horse anti-P. nigriventer immunoglobulins were prepared by immunoaffinity chromatography and used to set up a sandwich-type ELISA. The specificity of the assay was demonstrated by it...
A sandwich ELISA was then optimised using two distinct antibody combinations to detect sIRAP in the low nanogram range (16–500 ng/ml) with a sensitivity of 9 ng/ml and intra-assay variability < 10%. Importantly, the clinical validity of the ELISA was verified by the detection of ...
behaviourofSCSsandwichcompositewall basedonaseriesofcombinedcompressionanduniaxialbendingtestsonshortSCSsandwichcomposite wallwithinterlockingJ-hookconnectors.Fromthetests,itisfoundthattheSCSsandwichwallexhibits goodductilitybehaviourwithabendingfailuremode.Nonlinearfiniteelement(FE)modelisalsode- velopedtosimulatethe...
0748 Development and Application of Sandwich-ELISA for Direct Determination ofN. SHINOZAKI
A double antibody sandwich ELISA for rapid diagnosis of virus infection and to measure the humoral response against infectious bursal disease on clinical material. Avian Pathol. 27:450-454.Cardoso T C,Sousa R L,Alessi A C,et al.A double antibody sandwich ELISA for rapid diagnosis of virus ...
Eschbaumer M, Schultz C, Wackerlin R, Gauly M, Beer M, Hoffmann B (2011) Limitations of sandwich ELISAs for bluetongue virus antibody detection. Vet Rec 168: 643.Eschbaumer M, et al. Limitations of sandwich ELISAs for bluetongue virus antibody detection. Vet Rec 2011;168:643....
Sandwich ELISA assay for the quantitation of palytoxin and its analogs in natural samples. Boscolo S,Pelin M,De Bortoli M,et al. Environmental Science and Technology . 2013Boscolo S, Pelin M, De Bortoli M, Fontanive G, Barreras A, Berti F, Sosa S, Chaloin O, Bianco A, Yasumoto T, ...
This report shows that the use of a monoclonal antibody specific to the C-terminal extremity of mature PAP, combined with an anti-PAP polyclonal antibody did not detect PAPi, unless it was denaturated prior to ELISA. Thus, the specificity of this test was restricted to free PAP and the ...