52. Indirect and Sandwich ELISA是【中英字幕】可以练英语的生物化学课程之蛋白质简介2015 热力学和结构 分离纯化 测序分析 应用 (氨基酸、蛋白折叠;盐析、透析、SDS-PAGE、色谱)的第52集视频,该合集共计54集,视频收藏或关注UP主,及时了解更多相关视频内容。
HAZARI S;PANDA H K;KAR B C;.Compara tive evaluation of indirect and sandwich ELISA for the detection of antibodies to bovine respiratory syn- cytial virus (BRSV) in dairy cattle.Comp Immu- nol Microbiol Infect Dis.2002.59-68S Hazari,H K Panda,B C Kar,et al.Comparative evaluation of ...
And immunoglobulins(IgGs) purified from the immune sera was to developdouble sandwich ELISAfor detecting the antibodies of App serotype 2. 用胸膜肺炎放线杆菌血清2型(1536)菌液与弗氏不完全佐剂等体积混合乳化后免疫大白兔,制备兔高免血清,提纯抗体,建立检测猪传染性胸膜肺炎血清2型抗体的双夹心ELISA方法。
用辣根过氧化物酶标记的抗小鼠肝炎病毒(MHV)单克隆抗体(McAb),建立了ELISA 双抗体夹心法,并与 ELISA 间接法检测小鼠血清中的 MHV 抗体进行了比较。发现两种方法在检出率、特异性、稳定性方面并无明显差异,但由于间接法操作更为简便,因此认为,检测血清中 MHV 抗体采用间接法较好。
The PAL antigen-based ELISA assay was relatively simple to perform, precise, highly sensitive and specific, and reproducible. Based on our data the PAL antigen-based ELISA described here is the first indirect sandwich ELISA for urinary antigen detection which could easily be applied for diagnosis ...
Development of Double-Antibody Sandwich Indirect ELISA to Detect Deer Blood鹿血双抗体夹心间接ELISA检测方法的建立鹿血ELISA多克隆抗体通过制备兔抗鹿血和小鼠抗鹿血多克隆抗体,建立了以兔抗为包被抗体,鼠抗为检测抗体的鹿血双抗体夹心间接ELISA检测方法。采用方阵滴定法确定了抗原、抗体的最佳反应浓度,并对ELISA各反应...
Common categories of ELISA: direct ELISA, indirect ELISA, sandwich ELISA (double antibody, double antigen), competitive ELISA. Disadvantages of Indirect ELISA The indirect ELISA method is similar to the direct method. The difference is that the primary antibody is not labeled with an enzyme. Instea...
At a cut-off of 13.2 PP the sensitivity of the IgG-sandwich ELISA was 100% and specificity 99.95%, while for the IgM-capture ELISA the values were 96.47 and 99.44%, respectively, at a cut-off of 7.1 PP. Compared to the virus neutralisation test, the IgG-sandwich ELISA was more ...
We offer a diverse range of highly specific monoclonal and polyclonal ELISA antibodies, perfect for detecting and quantifying proteins in your samples. Choose from various options, including primary, detection, and enzyme-conjugated antibodies, as well as optimized kits for sandwich ELISA. Click the ...
Detailed information about indirect ELISA application in the determination of antibody titer and procedures of antibody concentration determination are discussed in the following section of ELISA applications. Learn more: Indirect ELISA 丨 Direct ELISA丨Sandwich ELISA丨Competitive ELISA丨ELISPOT...