52. Indirect and Sandwich ELISA是【中英字幕】可以练英语的生物化学课程之蛋白质简介2015 热力学和结构 分离纯化 测序分析 应用 (氨基酸、蛋白折叠;盐析、透析、SDS-PAGE、色谱)的第52集视频,该合集共计54集,视频收藏或关注UP主,及时了解更多相关视频内容。
用辣根过氧化物酶标记的抗小鼠肝炎病毒(MHV)单克隆抗体(McAb),建立了ELISA 双抗体夹心法,并与 ELISA 间接法检测小鼠血清中的 MHV 抗体进行了比较。发现两种方法在检出率、特异性、稳定性方面并无明显差异,但由于间接法操作更为简便,因此认为,检测血清中 MHV 抗体采用间接法较好。
Results The sensitivities of three indirect ELISA kits and a sandwich ELISA kit were 90. 2%,78. 0%,95. 1%,and 97. 6%,respectively,and the specificities were 78. 1%,72. 6%,94. 1%,and 100%,respectively. The sandwich ELISA kit had a 4-8 times higher sensitivity than indirect ELISA ...
Development of Double-Antibody Sandwich Indirect ELISA to Detect Deer Blood鹿血双抗体夹心间接ELISA检测方法的建立鹿血ELISA多克隆抗体通过制备兔抗鹿血和小鼠抗鹿血多克隆抗体,建立了以兔抗为包被抗体,鼠抗为检测抗体的鹿血双抗体夹心间接ELISA检测方法。采用方阵滴定法确定了抗原、抗体的最佳反应浓度,并对ELISA各反应...
To determine the lowest detection range in an indirect ELISA of one of the antibodies, RB9 was tested against decreasing purified sIRAP concentrations. Binding of RB9 to sIRAP was detectable in the nanogram range (0.001 µg/well; Fig. 2c). Notably, RB9 produced equivalent readings between pur...
Based on our data the PAL antigen-based ELISA described here is the first indirect sandwich ELISA for urinary antigen detection which could easily be applied for diagnosis of Legionnaires disease. 展开 关键词: Legionella Legionnaires disease (LD Peptidoglycan-associated lipoprotein (PAL ELISA EIA ...
方法在制备高滴度布氏菌抗原辣根过氧化物酶结合物及其冻干制品基础上,应用酶联免疫吸附双抗原夹心法即双抗原夹心酶联免疫吸附试验(DAgS-ELISA)、试管凝集试验(SAT)及虎红平板凝集试验(RBPT)对从山西省布氏菌病疫区收集的布氏菌病患者、布氏菌感染羊、牛、猪以及健康人、羊、牛、猪共计290份血清进行对比检测。
1. Development of double-antibody sandwich indirect ELISA to detect pseudorabies virus; 检测伪狂犬病病毒双抗体夹心间接ELISA方法的建立与应用2) double sandwich 双夹心间接ELISA3) double antibody ABC-ELISA 双抗体夹心ABC-ELISA4) double antibody sandwich ELISA 双抗体夹心ELISA 1. Development of ...
Optimization, comparison, and application of colorimetric vs. chemiluminescence based indirect sandwich ELISA for measurement of human IL-23. Optimization, comparison, and application of colorimetric vs. chemiluminescence based indirect sandwich ELISA for measurement of human IL-23. J Immunoassay ......
The aims of this study were identified of avian reovirus (ARV) from the chickens with tenosynovitis by indirectsandwich-enzyme linked immunosorbent assay (is-ELISA), and analysis of protein virus by sodium dodecyl sulphate(SDS-PAGE), and characterized of protein by western blot. Several chickens ...