酶底物产品名称优势、规格# 用于检测*吸收光波长(nm)颜色检测限***Recommended primary (1°) and secondary (2°) antibody dilutions**货号 Alkaline phosphatase (AP) PNPP (p-Nitrophenyl Phosphate) 1-Step PNPP 底物溶液 即用型规格 1,000 孔 405 黄色 100 ng/mL 1°—1:500 2°—1:...
Primary and Secondary Antibodies Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. Buy a primary antibody and get asecondar...
4. The secondary antibody, specific to the primary antibody is added. This second antibody is coupled to the enzyme 21、. 5. A substrate is added, and remaining enzymes elicit a chromogenic or fluorescent signal. ELISAELISA实验过程实验过程1. 实验试剂的准备 按试剂盒说明书的要求准备实验中需用...
Novel coronavirus antibodies, proteins and kits FeaturedProducts Anti-ACTB antibody (1-80 aa) (STJ91464) Clonality : Polyclonal Host : Rabbit Applications : IF/WB/IHC/ELISA Reactivity : Human/Mouse/Rat/Chicken/Globefish/Cow/Hamster/Pig/Sheep/Cat/Pig/Dog/Sheep ...
The secondary antibody has specificity for the primary antibody. In a sandwich ELISA, it is critical that the secondary antibody is specific for the detection of the primary antibody only (and not the capture antibody) or the assay will not be ...
Abbexa is based at the World renowned Cambridge Science Park , in Cambridge , UK . We are a supplier and distributor of biological toolsfor the life science , pharmaceutical development and biotechnology sectors . We provide the scientific...
Direct ELISA, where the antigen is immobilized on the ELISA plate, and the primary antibody carries the label (Figure 1 A) Indirect ELISA, where the antigen is immobilized on the ELISA plate, and the secondary antibody carries the label (Figure 1 B) Sandwich ELISA, where two primary ant...
Anticardiolipin antibodySystemic lupus erythematosusThrombosisAntiphosphatidylserine antibodiesThe clinical significance of anti-beta 2 glycoprotein I (beta 2-GPI) antibodies was evaluated in patients with antiphospholipid syndrome (APS), primary and secondary to systemic lupus erythematosus (SLE). Anti-beta ...
Direct ELISA, where the antigen is immobilized on the ELISA plate, and the primary antibody carries the label (Figure 1 A) Indirect ELISA, where the antigen is immobilized on the ELISA plate, and the secondary antibody carries the label (Figure 1 B) Sandwich ELISA, where two primary ant...
Coating antibody (1-10 μg/ml in coating buffer) Samples, standards and controls Primary antibody (unlabeled or biotinylated) Enzyme labeled secondary antibody Substrate Coating buffer: 0.15 M sodium carbonate, 0.35 M sodium bicarbonate, pH 9.6 ...