4.Afterwards, a secondary antibody is added, which will bind any antibody produced by a member of the donors species (for example, an antibody produced in a mouse that will bind any rabbit antibody). This secondar 11、y antibody often has an enzyme attached to it, which has no effect on...
it is critical that the secondary antibody is specific for the detection of the primary antibody only (and not the capture antibody) or the assay will not be specific for the antigen. Generally, this is achieved by using capture and primary antibod...
Versatile because many primary antibodies can be made in one species and the same labeled secondary antibody can be used for detection. Maximum immunoreactivity of the primary antibody is retained because it is not labeled. Sensitivity is increased because...
Direct ELISAs use a conjugated primary antibody, while indirect ELISAs include an additional amplification step. In an indirect ELISA, an unconjugated primary antibody binds to the antigen, then a labeled secondary antibody directed against the host species of the primary antibody binds to the ...
by using capture and primary antibodies from different host species (e.g., mouse IgG and rabbit IgG, respectively). For sandwich assays, it is beneficial to use secondary antibodies that have beencross-adsorbedto remove any secondary antibodies that m...
Versatile because many primary antibodies can be made in one species and the same labeled secondary antibody can be used for detection. Maximum immunoreactivity of the primary antibody is retained because it is not labeled. Sensitivity is increased because...
Afterwards,asecondaryantibodyisadded,whichwill bind any antibody produced by a member of the donor's species (for example, an antibody produced in a mouse that will bind any rabbit antibody). This secondary antibody often has an enzyme attached to it, which has no effect on the binding propert...
High specificity, selected antibody pairs Antibodies 13,000+ monoclonal & polyclonal primary antibodies; Various tagged secondary antibodies like HRP/FITC/Biotin; Validated Image for IHC, WB, IF, FC...etc; Custom Antibody Production Proteins
Secondary Antibodies Conjugated, unconjugated secondaries, ... ELISA & Assay Kits ELISA Kits, CLIA Kits, ELISpot, Assays, ... Proteins Native and recombinant, active, full length or partial proteins, ... Peptides Blocking peptides, conjugated, synthetic, ... Arrays Antibody Arrays, Pepti...
(enzyme-labeled secondary antibody). The detection antibodies are usually labeled with alkaline phosphatase (AP) or horseradish peroxidase (HRP). A large selection of substrates is available for performing the ELISA with an HRP or AP conjugate. The choice of substrate depends upon the required ...