Perhaps you are interested in a particular antibody for your research but don’t want to get a large quantity of it right out of the gate. We completely understand, and it’s why we’ve given the option of trial
Secondary (indirect) immunofluorescence uses two antibodies;the unlabeled first (primary) antibody specifically binds the target molecule, and the secondary antibody, which carries the fluorophore, recognizes the primary antibody and binds to it. Multiple secondary antibodies can bind a single primary anti...
What is the purpose of the enzyme conjugated to the secondary antibody in the ELISA test? Why are many injections given in the hypodermis? Why is skin resistance a factor in EMG? Explain. Why is the production of reduced coenzymes important in the TCA cycle? Why do single sugars tend to...
Explain the primary and secondary immune response to an antigen. Describe how an extracellular antigen is presented to a B cell and a CD4^+ T cell in order to initiate an immune response. Why do we always need to be sure the antibody used for detecting a pathogen is ...
Direct secondary antibody staining, or staining with a polyclonal pool from the same species as the primary, are sometimes used as negative controls. However, neither is likely to match the primary antibody isotype or replicate primary antibody binding patterns in the tissue as well as an isotype...
Next, the membranes were incubated with an HRP-conjugated secondary antibody for 2 h at room temperature. β-Actin was used as an internal control for normalizing relative protein levels. ECL reagent was added for film development. The film was scanned, and the optical density values of the ...
What role do cytotoxic T cells play in fighting infection? (a) Explain the primary and secondary antibody response. (b) What type of cells is responsible for each of these responses? Explain antigens. How are they related to an antibody? H...
Explain why the Ebola virus, which is highly virulent, is less common than the flu virus, which is not as virulent. Explain why a secondary antibody response to an antigen may prevent a bacterial or viral disease when the primary adaptive immu...