1.Yang Shi et.al, RNA m6A methylation across the transcriptome.Molecular Cell 83, February 2, 2023. 2.Matthias David Erlacheri et.al, Translating the epitranscriptome.WIREs RNA 2017, 8:e1375. doi: 10.1002/wrna.1375. 3.Xiaoke Guo et.al, The development of small molecules targeting methyltra...
选择性烯丙基化学标记和测序(m6A-SAC-seq)可以直接标记m6A,覆盖几乎所有的m6A经典基序,并以单碱基分辨率定量分析捕获的m6A位点(Hu等,2022)。这种方法利用特定的酶在m6A上加一个烯丙基化学基团,形成a6m6A,经I2处理后发生环化,并在随后的逆转录过程中被逆转录酶读为突变。基于突变位点检测转录组中m6A的位置,并通过...
m6A RNA modifications are measured at single-base resolution across the mammalian transcriptome Lulu Hu, Shun Liu, Yong Peng, Ruiqi Ge, Rui Su, Chamara Senevirathne, Bryan T. Harada, Qing Dai, Jiangbo Wei, Lisheng Zhang, Ziyang Hao, ...
Transcriptome-wide analysis of RNA m6A methylation regulation of muscle development in Queshan Black pigsQueshan Black pigLongissimus dorsiMeRIP-SeqMuscle growth and developmentm6A-modified genesN6-methyladenosine (m6A) refers to the methylation modification of N6position of RNA adenine, a dynamic ...
Comprehensive analyses of m6A regulators and interactive coding and non-coding RNAs across 32 cancer types. Mol. Cancer 20, 67 (2021). Article CAS PubMed PubMed Central Google Scholar An, Y. & Duan, H. The role of m6A RNA methylation in cancer metabolism. Mol. Cancer 21, 14 (2022)....
据了解,因无法在整个转录组中绘制单个m6A修饰位点,此前,RNA m6A修饰的功能研究受到阻碍。 附:英文原文 Title: m6A RNA modifications are measured at single-base resolution across the mammalian transcriptome Author: Hu, Lulu, Liu, Shun, Peng, Yong, Ge, Ruiqi, Su, Rui, Senevirathne, Chamara, Harada...
However, the regulatory mechanisms of m6A formation and the function of m6A in regulating cell reprogramming are still largely unknown. Here we examined the transcriptome-wide distribution of m6A modification in mouse ESCs, induced pluripotent stem cells (iPSCs), neural stem cells (NSCs), and ...
& He, C. Where, when, and how: context-dependent functions of RNA methylation writers, readers, and erasers. Mol. Cell 74, 640–650 (2019). Article CAS PubMed PubMed Central Google Scholar Patil, D. P., Pickering, B. F. & Jaffrey, S. R. Reading m6A in the transcriptome: m6A-...
RNA m6A methylation orchestrates cancer growth and metastasis via macrophage reprogramming Huilong Yin, Xiang Zhang, Pengyuan Yang, Xiaofang Zhang, Yingran Peng, Da Li, Yanping Yu, Ye Wu, Yidi Wang, Jinbao Zhang, Xiaochen Ding, Xiangpeng Wang, Angang Yang & Rui Zhang Natur...
Direct RNA Sequencing(DRS)基于Nanopore测序平台,不经反转录、无需扩增,直接读取全长转录本,无测序偏好性。可以检测RNA分子上的(m6A、m5C和假尿苷)等甲基化修饰位点;能准确分析可变剪切、融合基因和鉴定新转录本;此外,还可对Poly(A)尾长度和位点进行相对准确的估算和预测,还原真实RNA特征。