Despite recent advances in m7G research, the locations and functions of internal m7G modifications remain largely unknown. Internal m7G modifications have been suggested to be widely associated with the dysregulations of disease-related proteins, implicating internal m7G methylation as a novelepitranscriptome...
are proteins with significantly increased concentration after the RNA incubation.cColour-coded label-free quantitation (LFQ) intensities of proteins affected by the high structured (HS) RNA on a scale from black (low) to red (high). Hierarchical clustering by Perseus is indicated. For comparison,...
Here, we focus on the discovery of m6A readers (proteins that specifically recognize m6A), and their functions in tuning mRNA stability, as well as the broader significance of such m6A-dependent regulation of gene expression.关键词: RNA methylation m(6)A N-6-methyladenosine reversible RNA ...
In agreement with the dynamic expression of the In parallel to the methylation analysis using the m6 m6 6 A A-related proteins, the global m A levels of poly(A) peaks identified using exomePeak, we re-analyzed the RNA also decreased from P7 to P60 (Fig. 3d). The m6 highest m6 6 ...
Ribose 2′-O-methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5. Nat. Immunol. 12, 137–143 (2011). Article PubMed PubMed Central Google Scholar Galloway, A. & Cowling, V. H. mRNA cap regulation in mammalian cell...
Wang X, He C. 2014. Reading RNA methylation codes through methyl-specific binding proteins. RNA Biology 11:669-672. doi: 10.4161/rna.28829, PMID: 24823649Wang X, He C. Reading RNA methylation codes through methyl-specific binding proteins. RNA Biol. 2014; 11: 669-72. https://doi.org/...
另一研究使用LNCaP细胞系说明miR-106b/miR-25能通过靶向caspase 7凋亡相关半胱氨酸肽酶(caspase 7, apoptosis-related cysteine peptidase, CASP7)的mRNA调节前列腺癌进展,该酶在原位和转移性前列腺癌病灶中均存在下调[134]。总之,miR-106b/miR-25簇是一种致癌miR簇,其表达水平往往在PIN、原位、转移性前列腺癌中...
3.Shibutani ST, Saitoh T, Nowag H, Munz C, Yoshimori T. Autophagy and autophagy-related proteins in the immune system.Nat. Immunol.2015;16:1014–1024. 4.Rubinsztein DC, Marino G, Kroemer G. Autophagy and aging.Cell.2011;146:682–695. ...
基于circRNA的m6A甲基化富集倍数和表达量,我们可以分别鉴定出差异表达的circRNA(DEC,Differential Expression circRNA)和差异甲基化的circRNA(DMC,Differential Methylation circRNAs)。获得DEC、DMC信息后,即可开展维恩、占比、四九象限图的多角度分析(图5),详细讨论circRNA甲基化差异与表达量差异的趋势变化,潜在关联。
Methylation of ERV mRNAs is catalysed by the complex of methyltransferase-like METTL3–METTL144 proteins, and we found that depletion of METTL3–METTL14, along with their accessory subunits WTAP and ZC3H13, led to increased mRNA abundance of intracisternal A-particles (IAPs) and related ERVK ...