As each method has its own unique features, the goal of this review is to describe how different proximity labeling methods can be used to answer different biological questions. This article is categorized under: Technologies > Analysis of Proteins....
RNA molecules are highly compartmentalized in eukaryotic cells, with their localizations intimately linked to their functions. Despite the importance of RNA targeting, our current knowledge of the spatial organization of the transcriptome has been limite
Proximity-Dependent Labeling of Cysteines. Mapping protein-protein interactions is crucial for understanding various signaling pathways in living cells, and developing new techniques for this purpose has attracted significant interest. Classic methods (e.g., the yeast two-hybrid)... S Sen,N Sultana,...
APEX2, an engineered ascorbate peroxidase that enables proximity‐dependent labeling of proteins in living cells, has emerged as a powerful tool for deciphering the molecular architecture of various subcellular structures. However, only phenolic compounds have thus far been employed as APEX2 substrates,...
The development of proximity labeling technology and its applications in mammals, plants, and microorganisms Jieyu Guo Shuang Guo Wu Liu Cell Communication and Signaling (2023) Establishment of in vivo proximity labeling with biotin using TurboID in the filamentous fungus Sordaria macrospora Lucas S...
Mapping C. difficile TcdB interactions with host cell-surface and intracellular factors using proximity-dependent biotinylation labeling Bacterial toxins are the causative agents of many human diseases. Further characterizing the intoxication mechanisms of these proteins is important for the development of ...
Proximity labeling: an emerging tool for probing in planta molecular interactions 2021, Plant Communications Citation Excerpt : It should be noted that the optimal temperature for the catalytic activity of both BioID and BioID2 is 37°C, which may cause heat stress in plant cells, given that ...
Proximity labeling catalyzed by promiscuous enzymes, such as TurboID, have enabled the proteomic analysis of subcellular regions difficult or impossible to access by conventional fractionation-based approaches. Yet some cellular regions, such as organelle contact sites, remain out of reach for current PL...
Proximity labeling (PL) catalyzed by promiscuous enzymes such as TurboID have enabled the proteomic analysis of subcellular regions difficult or impossible to access by conventional fractionation-based approaches. Yet some cellular regions, such as organelle contact sites, remain out of reach for current...
Enzymatic-based proximity labeling approaches based on activated esters or phenoxy radicals have been widely used for mapping subcellular proteome and protein interactors in living cells. However, activated esters are poorly reactive which leads to a wid