Proximity-dependent labelingWe have developed protein chemical labeling method via single-electron-transfer (SET) mediated radical reaction that proceeds in close proximity to ruthenium photocatalyst. Photoactivated ruthenium photocatalyst oxidizes tyrosine residue or/and labeling reagents by SET.Michihiko ...
Proximity-dependent labeling has proven to be a valuable method for revealing protein-protein interaction networks in living cells. A mutant form of the biotin protein ligase enzyme from Aquifex aeolicus (BioID2) underpins this methodology by producing biotin that is attached to proteins that enter ...
RNA molecules are highly compartmentalized in eukaryotic cells, with their localizations intimately linked to their functions. Despite the importance of RNA targeting, our current knowledge of the spatial organization of the transcriptome has been limite
The development of proximity labeling technology and its applications in mammals, plants, and microorganisms Jieyu Guo Shuang Guo Wu Liu Cell Communication and Signaling(2023) Establishment of in vivo proximity labeling with biotin using TurboID in the filamentous fungus Sordaria macrospora ...
Chen Y, Gu M, Gunning PW, Russell SM. Dense small molecule labeling enables activator-dependent STORM by proximity mapping. Histochem Cell Biol. 2016; 146: 255-266. https://doi.org/10.1007/ s00418-016-1451-6 PMID: 27246003Chen Y, Gu M, Gunning PW, Russels SM (2016) Dense small mol...
Thus, our study shows that proximity labeling proteomics can be applied in male germ cells, although the choice of biotin ligase needs to be carefully tested.doi:10.1038/s41598-022-26501-7Seiya Ouragrid.136593.b0000 0004 0373 3971Department of Experimental Genome ResearchResearch Institute for ...
International Journal of Molecular Sciences Review Mapping the SARS-CoV-2–Host Protein–Protein Interactome by Affinity Purification Mass Spectrometry and Proximity-Dependent Biotin Labeling: A Rational and Straightforward Route to Discover Host-Directed Anti-SARS-CoV-2 Therapeutics Rosa Terracciano 1,*,...
4.3. Proximity-Dependent Protein Labeling Methods—Sample Preparation for Mass Spectrometry myc-BirA*-eIF3A and myc-BirA* vectors were transiently transfected into the cells using Lipofectamin LTX with PLUSTM reagent (Thermo Scientific, San Jose, CA, USA) in Biotin free MEM with Earle's salts ...
APEX2, an engineered ascorbate peroxidase that enables proximity‐dependent labeling of proteins in living cells, has emerged as a powerful tool for deciphering the molecular architecture of various subcellular structures. However, only phenolic compounds have thus far been employed as APEX2 substrates,...
Monomeric StreptavidinBiotinFluorescent LabelingDissociation RateProximity-Dependent BiotinylationBecause streptavidin is a homotetramer, it can bind multiple biotinylated ligands and cause target aggregation. To allow biotin detection without clustering, we previously engineered monomeric streptavidin (mSA) that is...