Using a proximity-dependent labeling system, we identified distinct classes of proteins that regulate R-loops in vivo through different mechanisms. We show that ATRX suppresses R-loops by interacting with RNAs and preventing R-loop formation. Our proteomics screen also discovered an unexpected ...
Proximity-dependent labeling techniques utilize enzymes fused to a protein of interest (POI) that can transfer a molecular tag to proteins situated within a suitable labeling radius in the cell [1], [2], [3]. The molecular tag facilitates enrichment and identification of the labeled species, ...
Proximity-dependent labeling has proven to be a valuable method for revealing protein-protein interaction networks in living cells. A mutant form of the biotin protein ligase enzyme from Aquifex aeolicus (BioID2) underpins this methodology by producing biotin that is attached to proteins that enter ...
Overall, the present study demonstrates that proximity-dependent labeling methods in living cells are suitable to get an insight of invadopodia molecular composition. Indeed, we identified known invadopodia components/regulators and new potential invadopodia players for which validation of their implication i...
To that end, we employed the proximity-dependent labeling technique BioID. We first generated a stable cell line that constitutively expressed the IGF1R as a fusion with BirA* (IGF1R–BirA*). To validate our stable cell line, we first performed immunofluorescence. IGF1R-BirA* was expressed ...
Graph: Fig 2 GFP nanobody-directed TurboID as an improved method for proximity-dependent labeling.(A) Schematic of indirect TurboID method, whereby the bio... E Holzer,C Rumpf-Kienzl,S Falk,... - 《Plos Genetics》 被引量: 0发表: 2023年 When less is more – a fast TurboID knock-in ...
BioID: A Proximity-Dependent Labeling Approach in Proteomics Study Peipei Liet al.,2019,Springer Protocols Proteomic Analysis of SUMOylation in the Post-ischemic Brain J. Will Thompsonet al.,2017,Springer Protocols SH2 Binding Site Protection Assay: A Method for Identification of SH2 Domain Intera...
Split-TurboID enables contact-dependent proximity labeling in cells Proximity labeling catalyzed by promiscuous enzymes, such as TurboID, have enabled the proteomic analysis of subcellular regions difficult or impossible to... KF Cho,TC Branon,S Rajeev,... - 《Proceedings of the National Academy of...
A proximity-dependent biotinylation map of a human cell. Nature 595, 120–124 (2021). Article CAS PubMed Google Scholar Prikas, E., Poljak, A. & Ittner, A. Mapping p38α mitogen-activated protein kinase signaling by proximity-dependent labeling. Protein Sci. 29, 1196–1210 (2020). ...
Nevertheless, both datasets identified a strong correlation between SG-proximal RNA labeling and transcript length, suggesting that longer RNAs are more likely localized to the SG. More recently, Kleiner and co-workers applied proximity-dependent RNA editing technique (TRIBE-ID) to profile G3BP1-...