Proximity-dependent labelingWe have developed protein chemical labeling method via single-electron-transfer (SET) mediated radical reaction that proceeds in close proximity to ruthenium photocatalyst. Photoactivated ruthenium photocatalyst oxidizes tyrosine residue or/and labeling reagents by SET.Michihiko ...
TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity. Nat. Commun.10, 3252 (2019). Larochelle, M., Bergeron, D., Arcand, B. & Bachand, F. Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction ...
Proximity-dependent labeling methods for proteomic profiling in living cells: an update Wiley Inter. Rev. Dev. Biol., 10 (2021), p. e392 View in ScopusGoogle Scholar 3 S.O. Obado, M.C. Field, B.T. Chait, M.P. Rout High-efficiency isolation of nuclear envelope protein complexes from...
APEX2, an engineered ascorbate peroxidase that enables proximity‐dependent labeling of proteins in living cells, has emerged as a powerful tool for deciphering the molecular architecture of various subcellular structures. However, only phenolic compounds have thus far been employed as APEX2 substrates,...
RNA molecules are highly compartmentalized in eukaryotic cells, with their localizations intimately linked to their functions. Despite the importance of RNA targeting, our current knowledge of the spatial organization of the transcriptome has been limite
Proximity-Dependent Labeling of Cysteines. Mapping protein-protein interactions is crucial for understanding various signaling pathways in living cells, and developing new techniques for this purpose has attracted significant interest. Classic methods (e.g., the yeast two-hybrid)... S Sen,N Sultana,...
A proximity-dependent biotinylation map of a human cell. Nature 595, 120–124 (2021). Article CAS PubMed Google Scholar Prikas, E., Poljak, A. & Ittner, A. Mapping p38α mitogen-activated protein kinase signaling by proximity-dependent labeling. Protein Sci. 29, 1196–1210 (2020). ...
Wang, P. et al. Mapping spatial transcriptome with light-activated proximity-dependent RNA labeling.Nat. Chem. Biol.15, 1110–1119 (2019). ArticleCASPubMedGoogle Scholar McHugh, C. A. & Guttman, M. RAP-MS: a method to identify proteins that interact directly with a specific RNA molecule...
Filling the void: Proximity-based labeling of proteins in living cells Trends Cell Biol., 26 (2016), pp. 804-817 View PDFView articleView in ScopusGoogle Scholar 3 R. Varnaitė, S.A. MacNeill Meet the neighbors: Mapping local protein interactomes by proximity-dependent labeling with BioID...
Nevertheless, both datasets identified a strong correlation between SG-proximal RNA labeling and transcript length, suggesting that longer RNAs are more likely localized to the SG. More recently, Kleiner and co-workers applied proximity-dependent RNA editing technique (TRIBE-ID) to profile G3BP1-...