BIOTINTRANSGENIC micePROTEINSPROTEIN-protein interactionsMEIOSISCharacterization of protein–protein interactions (PPI) is a key to understanding the functions of proteins of interest. Recently developed proximity-dependent biotin identification (BioID) has been actively investigated as an alternative PPI ...
N. An actin-dependent step in mitochondrial fission mediated by the ER-associated formin INF2. Science 339, 464–467 (2013). Article CAS PubMed ADS Google Scholar Bersuker, K. et al. A proximity labeling strategy provides insights into the composition and dynamics of lipid droplet proteome...
Current proximity-dependent labeling techniques include tyramide (15), ascorbate peroxidase (APEX) (16, 17), and proximity-dependent biotin identification (BioID) (14). Proximity-dependent labeling techniques such as BioID have been making a major impact on our understanding of protein interaction ...
Meet the neighbors: Mapping local protein interactomes by proximity‐dependent labeling with BioID Proximity-dependent biotin identification (BioID) is a recently developed method that allows the identification of proteins in the close vicinity of a prot... R Varnaitė,SA Macneill - 《Proteomics》...
We include instructions for clonal cell line generation, live biotin labeling of proximal proteins, isolation of biotinylated proteins, and sample processing for proteomic analysis using the biotin ligase miniTurbo with wild-type and mutant PKAc.1,2 For complete details on the use and execution of...
APEX2, an engineered ascorbate peroxidase that enables proximity‐dependent labeling of proteins in living cells, has emerged as a powerful tool for deciphering the molecular architecture of various subcellular structures. However, only phenolic compounds have thus far been employed as APEX2 substrates,...
Cho, K. F. et al. Split-TurboID enables contact-dependent proximity labeling in cells.Proc. Natl Acad. Sci. USA117, 12143–12154 (2020). Udeshi, N. D. et al. Antibodies to biotin enable large-scale detection of biotinylation sites on proteins.Nat. Methods14, 1167–1170 (2017). ...
In this study, we employed the proximity-dependent biotin identification (BioID) approach that allows for the labeling of proximal proteins in living cells and their purification under harsh denaturing conditions [12, 80], to determine the interactome of detergent-insoluble poly-GR, -PR, and -GA...
Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate ...
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. J. Cell Biol. 196, 801–810 (2012). Article CAS Google Scholar Samavarchi-Tehrani, P., Samson, R. & Gingras, A.-C. Proximity dependent biotinylation: key enzymes and adaptation to ...