BIOTINTRANSGENIC micePROTEINSPROTEIN-protein interactionsMEIOSISCharacterization of protein–protein interactions (PPI) is a key to understanding the functions of proteins of interest. Recently developed proximity-dependent biotin identification (BioID) has been actively investigated as an alternative PPI ...
et al. Split-TurboID enables contact-dependent proximity labeling in cells. Proc. Natl Acad. Sci. USA117, 12143–12154 (2020). Udeshi, N. D. et al. Antibodies to biotin enable large-scale detection of biotinylation sites on proteins. Nat. Methods14, 1167–1170 (2017). Fazal, F. M...
Fig. 5: Analysis of the mitochondrial matrix and intermembrane space proteome via super-resolution proximity labeling with enhanced direct identification of biotinylation sites. a Schematic figure of biotin-labeling mitochondrial matrix, inner membrane, and intermembrane space proteome via proximity labeling...
Filling the void: Proximity-based labeling of proteins in living cells Trends Cell Biol., 26 (2016), pp. 804-817 View PDFView articleView in ScopusGoogle Scholar 3 R. Varnaitė, S.A. MacNeill Meet the neighbors: Mapping local protein interactomes by proximity-dependent labeling with BioID...
Zhang, Y., et al.TurboID-based proximity labeling reveals that UBR7 is a regulator of N NLR immune receptor-mediated immunity.Nature Communications.10(1), 3252 (2019). Arora, D., et al.Establishment of proximity-dependent biotinylation approaches in different plant model systems.bioRxiv. , (...
Current proximity-dependent labeling techniques include tyramide (15), ascorbate peroxidase (APEX) (16, 17), and proximity-dependent biotin identification (BioID) (14). Proximity-dependent labeling techniques such as BioID have been making a major impact on our understanding of protein interaction ...
APEX2, an engineered ascorbate peroxidase that enables proximity‐dependent labeling of proteins in living cells, has emerged as a powerful tool for deciphering the molecular architecture of various subcellular structures. However, only phenolic compounds have thus far been employed as APEX2 substrates,...
Here we describe an alternative method based on proximity-dependent biotin identification (BioID), where specific integrin interaction partners are labeled in live cells and isolated without the need to isolate intact IACs. 展开 DOI: 10.1007/978-1-0716-0962-0_6 ...
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Fig. 1: Development and characterization of photoactivation-dependent proximity labeling method. a Schematic of miniSOG-mediated protein complex labeling. On blue light illumination, miniSOG-POI-expressing cells generate singlet oxygen, which modifies interacting proteins rather than non-binder proteins. Th...