Proximity‐dependent biotin identification (BioID) is quickly gaining popularity as a powerful tool for identifying novel protein‐protein and proximity‐based interactions in live cells. This technique relies on a promiscuous biotin ligase, which is fused to a protein of interest and, upon expression...
Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate ...
The identification of a bona fide lamin-like protein in Dictyostelium made this lower eukaryote an attractive model organism to study evolutionarily conserved nuclear envelope (NE) proteins important for nuclear organization and human laminopathies. Proximity-dependent biotin identification (BioID), reporte...
such as pyruvate kinase and glyceraldehyde-3-phosphate dehydrogenase. We have used Proximity-dependent biotin identification (BioID) technique to screen for transient or weak protein interactions of host proteins and viral replicase in vivo. We transiently expressed BirA* tagged TMV 126 kDa replicase...
Here we describe an alternative method based on proximity-dependent biotin identification (BioID), where specific integrin interaction partners are labeled in live cells and isolated without the need to isolate intact IACs. 展开 DOI: 10.1007/978-1-0716-0962-0_6 ...
To overcome those limitations, proximity-dependent biotin identification (BioID) technique can be used to identify transient or weak protein-protein interactionsin vivo[17,18]. It utilizes the mutant form of theEscherichia colibiotin ligase (BirA*, R118G) to tag biotin to proteins present in prox...
Batsios, P., Meyer, I. & Gräf, R. Proximity-Dependent Biotin Identification (BioID) inDictyostelium amoebae.Methods Enzymol.569, 23–42 (2016). ArticleCASPubMedGoogle Scholar Pitzen, V., Askarzada, S., Gräf, R. & Meyer, I. CDK5RAP2 is an essential scaffolding protein of the co...
Here we have used the proximity-dependent biotin identification (BioID) proximity proteomics approach to investigate the formation and collective composition of DPR aggregates using cellular models. While interactomes of arginine rich poly-GR and poly-PR aggregates overlapped and were enriched for ...
Proximity-dependent biotinylation techniques such as BioID provide an alternative approach to define the composition of cellular compartments in living cells5,6,7. Here we present a BioID-based map of a human cell on the basis of 192 subcellular markers, and define the intracellular locations of...
BioID is a non-toxic labelling systems based on the biotin ligase BirA that has been widely used in diverse living samples, including cultured cells18, mouse19, and yeast20. We previously developed ancestral BirA for proximity-dependent biotin identification (AirID) and showed that it has both...